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Multi-dimensional tomographic fluorescence microscopy imaging system and method based on polarization structured light modulation

A technology of microscopic imaging and polarization modulation, applied in microscopes, optics, optical components, etc., can solve the problem of reducing imaging speed and achieve the effect of less photobleaching

Active Publication Date: 2021-06-11
北京艾锐精仪科技有限公司
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Problems solved by technology

One of the solutions is to use fluorescent confocal microscopy, but this technique requires point scanning to achieve two-dimensional imaging, requiring hundreds of detections, which greatly reduces the imaging speed

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  • Multi-dimensional tomographic fluorescence microscopy imaging system and method based on polarization structured light modulation
  • Multi-dimensional tomographic fluorescence microscopy imaging system and method based on polarization structured light modulation
  • Multi-dimensional tomographic fluorescence microscopy imaging system and method based on polarization structured light modulation

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Embodiment Construction

[0065] The present invention will be further elaborated below through specific embodiments in conjunction with the accompanying drawings.

[0066] like figure 1As shown, the multidimensional tomographic fluorescence microscopy imaging system based on polarization structured light modulation in this embodiment includes: two lasers Ls1 and Ls2, a beam combiner, an acousto-optic tunable filter AOTF, a beam expander, and a polarization beam splitter PBS , half-wave plate HWP, spatial light modulator SLM, condenser lens L3, spatial filter Mk, vortex half-wave plate VHWP, first dichroic mirror DM1, first 4f system, second dichroic mirror DM2 , objective lens OB, emission filter EF, lens tube lens TL, second 4f system, aperture AP, band splitting device and camera C; wherein, each laser emits a linearly polarized laser of a band, and two lasers Ls1 and Ls2 Separately emit linearly polarized lasers of different wavelength bands; the first beam is reflected by the first mirror M1 and ...

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Abstract

The invention discloses a multi-dimensional tomographic fluorescence microscopic imaging system and method based on polarization structured light modulation. For the illumination of each polarization modulation direction, the present invention collects two phase-complementary sinusoidal structured light illumination patterns, obtains a uniform light illumination pattern by averaging the two sinusoidal structured light illumination patterns, and then combines the uniform light illumination pattern with Combining one of the sinusoidal structured light illumination patterns and using the HiLo algorithm can solve the optical tomographic image; only need to average the three structured light patterns to obtain a uniform light illumination pattern, and then combine with one of the structured light illumination patterns The HiLo algorithm can be used to solve the optical tomographic pattern; the invention adopts the method of simultaneous acquisition at the detection end, without adding additional acquisition time; the invention has great advantages in terms of imaging speed and less photobleaching.

Description

technical field [0001] The invention relates to the field of optical microscopic imaging, in particular to a multi-dimensional tomographic fluorescence microscopic imaging system and method based on polarization structured light modulation. Background technique [0002] Fluorescence microscopy imaging can perform non-invasive and specific observation of biological samples, which is of great significance in the research of subcellular structure and function. The basic physical properties of fluorescence include fluorescence intensity, polarization, spectrum, and fluorescence lifetime. Detecting these physical properties as much as possible can obtain more diverse information inside the cell. The method proposed by the invention can detect multidimensional information such as fluorescence intensity, spectrum and polarization. The present invention adopts polarization modulation illumination at the illumination end, through the modulation illumination of no less than three pol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/00G02B27/00
CPCG02B21/0076G02B27/0012
Inventor 席鹏张昊李美琪刘文辉戴琼海
Owner 北京艾锐精仪科技有限公司
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