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Xanthine oxidase inhibitory peptide

A technology of xanthine oxidase and inhibitory peptides, which is applied in the field of biomedicine, can solve the problems of limited reports on the types of XO inhibitors, and achieve good potential and application prospects

Inactive Publication Date: 2020-11-13
BOHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, in the prior art, the type reports for natural XO inhibitors are very limited, therefore, it is very meaningful to develop novel natural XO inhibitors

Method used

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Embodiment 1

[0031] This embodiment screens and identifies a tetrapeptide EEAK that has a significant inhibitory effect on xanthine oxidase from tuna protein, including the following steps:

[0032] (1) Virtual enzymatic hydrolysis and preliminary screening of tuna protein. Based on the online program ExPASy PeptideCutter, tuna skeletal myosin weight was analyzed using typical gastrointestinal digestive proteases, pepsin (EC 3.4.23.1), trypsin (EC 3.4.21.4) and chymotrypsin (EC 3.4.21.1). The amino acid sequence of the tuna skeletal myosin heavy chain was obtained from the NCBI website (https: / / www.ncbi.nlm.nih.gov / ), and its number is BAA12730.1. Unreported tripeptides and tetrapeptides were selected for prediction of toxicity and water solubility properties using the online tools ToxinPred and Peptide propertycalculator. The non-toxic and soluble tripeptides and tetrapeptides were screened.

[0033] (2) Use DS software (full name Discovery Studio 2017 R2 Client) to carry out molecular ...

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Abstract

The invention particularly relates to a xanthine oxidase inhibitory peptide. The amino acid sequence of the xanthine oxidase inhibitory peptide is Glu-Glu-Ala-Lys (EEAK). The IC50 value of the tetrapeptide EEAK on xanthine oxidase inhibitory activity is 173.00+ / -0.06 [mu]M, the tetrapeptide EEAK has a continuous and stable inhibitory effect on xanthine oxidase, and the tetrapeptide EEAK has the outstanding advantages of safety, no toxic or side effect, easiness in absorption, industrialization and the like. The active tetrapeptide EEAK or the derivative thereof can be used for preparing gout treatment / prevention medicines or used as a functional food additive for long-term treatment and health care of gout patients, and has a wide application prospect and very important significance.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a xanthine oxidase inhibitory peptide. Background technique [0002] Gout is the second most common metabolic disease and can also cause complications such as hypertension, cardiovascular disease and diabetes. In addition, with the improvement of people's living standards, the prevalence of gout is increasing year by year, and the patients are getting younger, which has attracted people's attention. Therefore, it is very important to study anti-gout drugs, health food and inhibition mechanism. Xanthine oxidase (XO) can combine with xanthine and hypoxanthine to produce uric acid. Therefore, XO is considered to be a key target for the prevention and treatment of gout and related diseases. [0003] Synthetic XO inhibitors (such as allopurinol and febuxostat, etc.) Long-term use of these synthetic XO inhibitors can cause some toxic side effects, such as skin rashes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/113A61K38/07A61P19/06
CPCC07K5/1021A61P19/06A61K38/00
Inventor 于志鹏阚若彤赵文竹纪慧卓励建荣
Owner BOHAI UNIV
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