A multifunctional hybrid peptide with antibacterial, anti-inflammatory, endotoxin neutralizing and immunomodulatory activities, its preparation method and application

Abstract

The invention relates to the technical field of protein engineering, in particular to a multifunctional hybrid peptide with antibacterial, anti-inflammatory, endotoxin neutralizing and immunoregulatory activities, as well as its preparation method and application. The hybrid peptide provided by the present invention comprises: peptide I and peptide II fused with peptide I, wherein the amino acid sequence of peptide I is shown in SEQ ID NO.1, and the amino acid sequence of peptide II is shown in the 19th sequence of SEQ ID NO.2 ~ 29 shown. The hybrid peptide has the functions of antibacterial, endotoxin neutralization, immune regulation and anti-inflammation, and has the advantages of low cytotoxicity, high safety, convenient preparation and low cost, and can be used as an ideal antibacterial agent and endotoxin neutralizer , immunomodulator and anti-inflammatory agent, widely used in human and animal medicine, food, health care, feed, nutrition and other fields, with good application potential and value.

Description

technical field [0001] The invention relates to the technical field of protein engineering, in particular to a hybrid peptide with antibacterial, anti-inflammatory, endotoxin-neutralizing and immune-modulating activities, its preparation method and application. Background technique [0002] Bacterial infection is an infectious disease caused by pathogenic bacteria or conditional pathogenic bacteria invading the animal body to grow and reproduce, producing toxins (such as endotoxin) and other metabolites, which can induce inflammation and oxidative damage, etc., and damage the immune system in severe cases. even lead to death. When animals are young, weak, sick, stressed, or infected with pathogens, their immunity is often reduced, and then secondary infections (bacterial infections, etc.) occur, causing inflammatory reactions. For the treatment of infection and inflammation, the traditional control strategy is to use antibiotics and chemical drug agents. The continuous use...

AI Technical Summary

Problems solved by technology

[0006] However, natural bioactive peptides also have some defects, mainly as follows: (1) Some natural bioactive peptides have relatively low biological activity and single function, such as the problem of weak antibacterial activity of pig lactoferrin peptide ; and although cecropin A has good antibacterial efficacy, its anti-inflammatory ability is relatively weak; (2) Some natural bioactive peptides have high cytotoxicity, which can cause serious damage to the body while inhibiting and killing pathogenic microorganisms (such as melittin and LL-37)

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