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Xylaria striata strain and culture method of sclerotium of xylaria striata strain

A technology of vertical stripes and charcoal bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of late start of research

Inactive Publication Date: 2020-12-04
云南菌视界生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many types of fungi in the genus Carbonocera, but the research on this genus started relatively late, and 61 species have been reported so far

Method used

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  • Xylaria striata strain and culture method of sclerotium of xylaria striata strain
  • Xylaria striata strain and culture method of sclerotium of xylaria striata strain
  • Xylaria striata strain and culture method of sclerotium of xylaria striata strain

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Isolation and preservation of strains

[0027] In the ultra-clean workbench, inoculate the collected wild C. longitudinal stripes strains into enriched PDA medium by the tissue separation method, and cultivate them in the dark at a constant temperature of 25°C for 6 days. Observe and record every day, and remove the contaminated ones. Petri dish, select the petri dish with good growth as the mother species. Cut off the mycelium squares with a length and width of 0.8 cm, and store them in a centrifuge tube filled with sterile distilled water at 4°C.

[0028] Molecular Identification of Strain YK-4

[0029] Add the hyphae of YK-4 to the centrifuge tube and add an appropriate amount of liquid nitrogen to grind the mycelia; the extraction method refers to the steps of the fungal DNA extraction kit (new rapid plant genomic DNA extraction kit, Bio Take Corporation); PCR amplification: YK-4 The genomic DNA of -4 is used as a template, using forward primer ITS4 (5'-TCCTCCGCTT...

Embodiment 2

[0048] Cultivation formula screening of sclerotia

[0049] Formula 1: miscellaneous sawdust 78%, soil 20%, sugar 1%, gypsum 1%

[0050] Formula 2: miscellaneous sawdust 78%, soil 10%, rice 5%, wheat bran 5%, sugar 1%, gypsum 1%

[0051] Formula 3: 98% rice medium, 1% gypsum, 1% sugar

[0052] Formula 4: 78% corn cob, 20% soil, 1% sugar, and 1% gypsum.

[0053] The culture materials of the 4 formulas were divided into bottles, each bottle was 6 cm high, and the average wet weight of each bottle was 100 g. At 121°C, autoclave for 2 hours, inoculate after cooling, and keep the inoculum volume consistent. Cultivation conditions Placed in an incubator for cultivation: the temperature is 25° C., cultivated in the dark, the moisture content of the compost is 65%, and the indoor cultivation humidity is 60%. The results showed that formulation 2 had the highest biological efficiency and the shortest growth maturation time (see Table 1 below).

[0054] Table 1:

[0055]

[0056...

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Abstract

The present invention belongs to the field of edible and medicinal fungi and particularly relates to a xylaria striata strain and a culture method of sclerotium of the xylaria striata strain. The sclerotium of the xylaria striata strain is rich in important components (mannitol and adenosine) for measuring medicinal value of xylaria in pharmacopeia, and the content of the important components is higher than that of many currently reported edible and medicinal fungi.

Description

technical field [0001] The invention belongs to the field of edible and medicinal fungi, and in particular relates to a strain of Xanthomonas longitudinal stripes and a method for cultivating the sclerotium. Background technique [0002] Xylaria striata belongs to Xylariaceae, Xylaria genus fungi[1]-[3], Southwest University of Science and Technology Huang Yi, Lei Chuanwen, Yuan Xiaohong and others applied for a patent in 2015 "A Application of Xylella vertical stripes" shows that the fruiting body or alcohol extract of Xylella vertical stripes has a significant sedative and hypnotic effect, and can be used to treat nervous system diseases such as insomnia, anxiety, or convulsions, with good results. Southwest University of Science and Technology Yuan Shijun, He Xinsheng, Yuan Xiaohong, etc. applied for a patent in 2019 "A method for producing the fruiting body of Xanthomonas vertical stripes by using liquid medium", and invented a method for directly producing the fruiting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14C12R2001/645C12N1/145
Inventor 李荣春闻绍峰李梦杰
Owner 云南菌视界生物科技有限公司
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