Xylaria striata strain and culture method of sclerotium of xylaria striata strain
A technology of vertical stripes and charcoal bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of late start of research
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Embodiment 1
[0026] Isolation and preservation of strains
[0027] In the ultra-clean workbench, inoculate the collected wild C. longitudinal stripes strains into enriched PDA medium by the tissue separation method, and cultivate them in the dark at a constant temperature of 25°C for 6 days. Observe and record every day, and remove the contaminated ones. Petri dish, select the petri dish with good growth as the mother species. Cut off the mycelium squares with a length and width of 0.8 cm, and store them in a centrifuge tube filled with sterile distilled water at 4°C.
[0028] Molecular Identification of Strain YK-4
[0029] Add the hyphae of YK-4 to the centrifuge tube and add an appropriate amount of liquid nitrogen to grind the mycelia; the extraction method refers to the steps of the fungal DNA extraction kit (new rapid plant genomic DNA extraction kit, Bio Take Corporation); PCR amplification: YK-4 The genomic DNA of -4 is used as a template, using forward primer ITS4 (5'-TCCTCCGCTT...
Embodiment 2
[0048] Cultivation formula screening of sclerotia
[0049] Formula 1: miscellaneous sawdust 78%, soil 20%, sugar 1%, gypsum 1%
[0050] Formula 2: miscellaneous sawdust 78%, soil 10%, rice 5%, wheat bran 5%, sugar 1%, gypsum 1%
[0051] Formula 3: 98% rice medium, 1% gypsum, 1% sugar
[0052] Formula 4: 78% corn cob, 20% soil, 1% sugar, and 1% gypsum.
[0053] The culture materials of the 4 formulas were divided into bottles, each bottle was 6 cm high, and the average wet weight of each bottle was 100 g. At 121°C, autoclave for 2 hours, inoculate after cooling, and keep the inoculum volume consistent. Cultivation conditions Placed in an incubator for cultivation: the temperature is 25° C., cultivated in the dark, the moisture content of the compost is 65%, and the indoor cultivation humidity is 60%. The results showed that formulation 2 had the highest biological efficiency and the shortest growth maturation time (see Table 1 below).
[0054] Table 1:
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