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Method for detecting pesticide residues in traditional Chinese medicinal materials

A technology for pesticide residues and traditional Chinese medicinal materials, applied in the field of pesticide detection, can solve the problems of inconsistent standards, low accuracy and poor repeatability of multi-residue detection, and achieve high accuracy and repeatability

Active Publication Date: 2020-12-04
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to the national standard 2763 and the Chinese Pharmacopoeia 2015, my country has set maximum residues for about 600 kinds of pesticides in agricultural products, but among Chinese medicinal materials, only 4 kinds of Chinese medicinal materials have stipulated a total of 7 kinds of pesticides mainly organochlorine pesticides Maximum residue limits, which lead to inconsistent standards between exporting countries and importing countries in the process of exporting traditional Chinese medicine, which has become a stumbling block for Chinese medicine to go global
[0004] The matrix of traditional Chinese medicinal materials is relatively complex, and the multi-residue detection of pesticides has the defects of low accuracy and poor repeatability

Method used

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  • Method for detecting pesticide residues in traditional Chinese medicinal materials
  • Method for detecting pesticide residues in traditional Chinese medicinal materials
  • Method for detecting pesticide residues in traditional Chinese medicinal materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] The Chinese medicinal material (Ophiopogon japonicus) to be tested was pulverized with a high-speed pulverizer and passed through a 40-mesh sieve for later use. Accurately weigh 2g of Chinese herbal medicine powder, add 10mL of water, vortex for 1min to obtain the first extraction product, add 6mL of 0.75% acetic acid acetonitrile, vortex for 1min, and ultrasonically extract for 15min to obtain the second extraction product, add 6g of anhydrous magnesium sulfate And 1.5g sodium chloride, shake vigorously for 1min. Centrifuge at 8000rpm for 5 minutes, use the supernatant as matrix extract, take 1mL of matrix extract, add 150mg of anhydrous magnesium sulfate and 5mg of nano-zinc oxide, vortex for 1min, centrifuge at 11000rpm for 5 minutes, take the supernatant and pass through a 0.22μm filter membrane Then add to the sample injection vial as the solution to be tested.

Embodiment 2

[0072] The Chinese medicinal material (Ophiopogon japonicus) to be tested was pulverized with a high-speed pulverizer and passed through a 40-mesh sieve for later use. Accurately weigh 2 g of Chinese herbal medicine powder, add 5 mL of water, vortex for 1 min, add 10 mL of 0.75% acetic acid acetonitrile, vortex for 1 min, ultrasonically extract for 15 min, add 6 g of anhydrous magnesium sulfate and 1.5 g of sodium chloride, and shake vigorously for 1 min. Centrifuge at 8000rpm for 5 minutes, use the supernatant as matrix extract, take 1mL of matrix extract, add 150mg of anhydrous magnesium sulfate and 5mg of nano-zinc oxide, vortex for 1min, centrifuge at 11000rpm for 5 minutes, take the supernatant and pass through a 0.22μm filter membrane Then add to the sample injection vial as the solution to be tested.

Embodiment 3

[0074] The Chinese medicinal material to be tested (Polygonatum Polygonatum) was pulverized with a high-speed pulverizer and passed through a 40-mesh sieve for later use. Accurately weigh 2g of Chinese herbal medicine powder, add 10mL of water, vortex for 1min to obtain the first extraction product, add 6mL of 0.75% acetic acid acetonitrile, vortex for 1min, and ultrasonically extract for 15min to obtain the second extraction product, add 6g of anhydrous magnesium sulfate And 1.5g sodium chloride, shake vigorously for 1min. Centrifuge at 8000rpm for 5 minutes, use the supernatant as matrix extract, take 1mL of matrix extract, add 150mg of anhydrous magnesium sulfate and 5mg of nano-zinc oxide, vortex for 1min, centrifuge at 11000rpm for 5 minutes, take the supernatant and pass through a 0.22μm filter membrane Then add to the sample injection vial as the solution to be tested.

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Abstract

The invention provides a method for detecting pesticide residues in traditional Chinese medicinal materials, and relates to the field of pesticide detection. The method comprises the following steps:mixing crushed traditional Chinese medicinal materials to be detected with water, and carrying out first extraction to obtain a first extraction product; mixing the first extraction product with an acetonitrile acetate aqueous solution, and carrying out second extraction to obtain a second extraction product; mixing the second extraction product with anhydrous magnesium sulfate and sodium chloride, and performing third extraction to obtain a third extraction product; centrifuging the third extraction product to obtain a supernatant as a matrix extracting solution, mixing the matrix extractingsolution with a purifying agent, carrying out purification , and centrifuging a purified material to obtain a supernatant as a to-be-detected solution; and determining the to-be-determined liquid by using ultra-high performance liquid chromatography-tandem mass spectrometry. The detection method can realize simultaneous detection of up to 76 pesticides including insecticides, bactericides and plant growth regulators in the traditional Chinese medicinal materials, and has high accuracy and repeatability.

Description

technical field [0001] The present disclosure relates to the field of pesticide detection, in particular to a method for detecting pesticide residues in Chinese herbal medicines. Background technique [0002] Traditional Chinese medicine is one of the most important cultural heritages in my country, and it has the functions of health care, prevention and treatment of diseases. Nowadays, with the development of modern science and technology, traditional Chinese medicine is gradually going to the world. In recent years, the planting area of ​​traditional Chinese medicine in China has grown rapidly. There are more than 430 planting bases of common varieties, and the planting area is about 3.067 million hm 2 . In recent years, people have paid more and more attention to the safety of traditional Chinese medicine, because traditional Chinese medicine must not only ensure its efficacy, but also ensure its food safety. Some studies have pointed out that about 65-80% of people pr...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/062
Inventor 戴小枫范蓓孔志强李敏敏李瑞瑆金诺陈捷胤孙玉凤刘佳萌孙晶贾宁
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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