Aryl sulfatase and glucuronidase inhibitor, preparation method thereof and application of inhibitor
A technology of arylsulfatase and glucuronidase, which is applied in the medical field, can solve the problems of expensive inhibitors and high half-inhibitory concentrations, and achieve the effects of low price, good effect and simple operation
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Embodiment 1
[0026] Take two 3mL Tris-HCl buffer solution (pH=5.8) in two glass test tubes, denoted as test tube 1 and test tube 2. Proceed as follows for test tube 1 and test tube 2:
[0027] Test tube 1:
[0028] Add 0.212 U of arylsulfatase to test tube 1, and then pass through NO, so that the concentration of NO in the Tris-HCl buffer solution is 250 μg / L, and then add p-nitrophenol sulfuric acid conjugate according to the standard of 800 μmol / L. The glass test tube was placed in a water bath at 37°C for 1 hour, and then the reaction was terminated by adding 2 mL of 0.5 mol / L sodium hydroxide, using a standard method (refer to Tabatabai and Bremner, Soil Science Society of America Journal, 1970, 34: 225- 229) Determination of arylsulfatase activity.
[0029] Test tube 2:
[0030] Add 0.045U of glucuronidase to test tube 2, and then pass through NO, so that the concentration of NO in the Tris-HCl buffer solution is 250 μg / L, and then add p-nitrophenol glucuronide according to the sta...
Embodiment 2
[0032] Take two 3mL Tris-HCl buffer solution (pH=5.8) in two glass test tubes, denoted as test tube 1 and test tube 2. Proceed as follows for test tube 1 and test tube 2:
[0033] Test tube 1:
[0034] Add 0.212 U of arylsulfatase to test tube 1, and then pass through NO, so that the concentration of NO in the Tris-HCl buffer solution is 250 μg / L, and then add p-nitrophenol sulfuric acid conjugate according to the standard of 800 μmol / L. Place the glass test tube in a water bath at 37°C for 1 hour, then stop the reaction with 2 mL of 0.5 mol / L sodium hydroxide, and use a standard method (refer to Tabatabai and Bremner, Soil Science Society of America Journal, 1970, 34: 225-229 ) to measure the activity of arylsulfatase.
[0035] Test tube 2:
[0036] Add 0.045U of glucuronidase to test tube 2, and then pass through NO, so that the concentration of NO in the Tris-HCl buffer solution is 250 μg / L, and then add p-nitrophenol glucuronide according to the standard of 800 μmol / L. ...
Embodiment 3
[0038] Take two 3mL Tris-HCl buffer solution (pH=5.8) in two glass test tubes, denoted as test tube 1 and test tube 2. Proceed as follows for test tube 1 and test tube 2:
[0039] Test tube 1:
[0040] Add 0.212 U of arylsulfatase to test tube 1, then pass through NO, so that the concentration of NO in the Tris-HCl buffer solution is 500 μg / L, and then add p-nitrophenol sulfuric acid conjugate according to the standard of 800 μmol / L. Place the glass test tube in a water bath at 37°C for 1 hour, then stop the reaction with 2 mL of 0.5 mol / L sodium hydroxide, and use a standard method (refer to Tabatabai and Bremner, Soil Science Society of America Journal, 1970, 34: 225-229 ) to measure the activity of arylsulfatase.
[0041] Test tube 2:
[0042] Add 0.045U of glucuronidase to test tube 2, and then pass through NO, so that the concentration of NO in the Tris-HCl buffer solution is 500 μg / L, and then add p-nitrophenol glucuronide according to the standard of 800 μmol / L. bod...
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