Freeze-drying protective agent, fecal microbiota freeze-dried product and preparation method of freeze-drying protective agent
A technology of freeze-drying protective agent and fecal bacteria, which is applied in the field of freeze-drying protective agent, fecal bacteria freeze-dried products and their preparation, which can solve the problems of sensitive environmental conditions and difficult long-term survival, so as to improve storage temperature, protect cells, protect The effect of the freezing effect
Active Publication Date: 2021-01-08
SHENZHEN XBIOME BIOTECH CO LTD
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- Abstract
- Description
- Claims
- Application Information
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Problems solved by technology
Compared with the currently mature and commercialized lactic acid bacteria and probiotics, the microorganisms in the fecal flora are more anaerobic, many of which are sensitive to environmental conditions and difficult to survive in the laboratory environment for a long time
Method used
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Embodiment 1
[0031] A freeze-drying protectant composition, comprising the following components: 10% polydextrose, 1% mannitol, 1% glycine, 0.3% vitamin C and 0.01% Tween 80, and the balance is water.
Embodiment 2
[0033] A freeze-drying protectant composition, comprising the following components: 50% polydextrose, 5% mannitol, 5% glycine, 0.05% vitamin C and 1% Tween 80, and the balance is water.
Embodiment 3
[0035]A freeze-drying protectant composition comprises the following components: 30% polydextrose, 0.1% mannitol, 0.1% glycine, 0.01% vitamin C and 0.05% Tween 80, and the balance is water.
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The invention belongs to the field of microorganisms, and particularly relates to a freeze-drying protective agent, a fecal microbiota freeze-dried product and a preparation method of the freeze-drying protective agent. The freeze-drying protective agent comprises polydextrose, mannitol, glycine, vitamin C and Tween 80; the freeze-drying fecal microbiota product prepared from the freeze-drying protective agent is high in fecal microbiota total fungus survival rate and superior to trehalose in effect; and the potential risk caused by trehalose, maltodextrin and the like can be reduced, and thefinal curative effect of the fecal microbiota transplanting product can be potentially improved.
Description
technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a freeze-drying protective agent, a freeze-dried product of fecal bacteria and a preparation method thereof. Background technique [0002] Freeze-drying technology is a drying technology that freezes wet materials or solutions into a solid state at a relatively low temperature, and then directly sublimates the moisture in them into a gaseous state without going through a liquid state under a low vacuum degree, and finally dehydrates the material. Because freeze-drying is carried out at low temperature and low pressure, and the water is directly sublimated, it has many advantages in drying materials, such as drying heat-sensitive materials, and the dried materials are very stable and easy to store for a long time. Since the drying of the material is completed in a frozen state, compared with other drying methods, the physical structure and molecular structure of the...
Claims
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IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 张聪黄松高婷刘贵军施杰尹意铭谭验
Owner SHENZHEN XBIOME BIOTECH CO LTD
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