Method for analyzing distribution of secondary metabolites in gelsemium elegans in tissue
A technology for secondary metabolites and hooks, which is applied in the analysis of materials, material analysis by electromagnetic means, instruments, etc., can solve problems such as unclear spatial distribution, and achieve the effect of simple and convenient analysis and detection process.
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[0037] This example provides a method for analyzing the tissue distribution of secondary metabolites in geckos, including the following steps:
[0038] S1: Cut the cross-section of the hook kiss tissue, make a frozen section, and obtain a complete tissue sample imprint on the glass slide;
[0039] S2: Analyze the imprint of the tissue sample obtained in step S1 by a desorption electrospray ionization mass spectrometer to obtain a mass spectrometry imaging file;
[0040] S3: Process the mass spectrometry imaging file obtained in step S2 by image processing software, and obtain a visualized picture of the distribution of secondary metabolites in the tissue of the gecko.
[0041] In step S1, the method for making frozen sections is specifically:
[0042] Cut the cross-section of the glyphus tissue, which includes the roots, stems, leaves and buds of the glyphs, soak and fix the roots, stems, leaves, and buds of the glyphs in fresh tissue fixative solution, and fix them for 24 ho...
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