Tilapia calcium ion binding peptide as well as preparation method and application thereof

Abstract

The invention discloses a preparation method and application of tilapia calcium ion binding peptide. The preparation method comprises the following steps of pretreating tilapia processing wastes; then, adopting tilapia endogenous enzyme and commercial protease for compounding and enzymolysis; performing ultrafiltration separation; carrying out immobilized metal affinity chromatography; and preparing the active peptide and calcium ion conjugate to obtain the tilapia calcium ion binding peptide. The tilapia calcium ion binding peptide prepared by the method disclosed by the invention has biological activity of promoting calcium ion transport and absorption, and in addition, the preparation method of the tilapia calcium ion binding peptide disclosed by the invention is relatively simple in process and safe in product, and can be applied to actual production.

Description

technical field

[0001] The invention belongs to the technical field of mineral ion-binding peptides, and in particular relates to a tilapia calcium ion-binding peptide and its preparation method and application. Background technique

[0002] Peptide-mineral ion conjugate is an organic compound containing mineral ions, which is prepared by chelation reaction between mineral ions and peptides, which can improve the bioavailability of mineral ions by virtue of the absorption mechanism of peptides in the body , with physiological and biochemical properties that inorganic mineral ions do not have. At present, the chelate prepared by chelating essential trace elements and peptides has become a new type of mineral ion supplement, and has attracted more and more attention.

[0003] my country is a big producer of tilapia, and its output ranks first in the world. At present, the processing of tilapia is mainly processed into fish fillets. During this process, a large amount of proc...

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