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A kind of construction method of hplc fingerprint of germinal tablets

A fingerprint and construction method technology, which is applied in the field of traditional Chinese medicine analysis, can solve the problems of complex construction methods and inability to represent quality levels, and achieve high extraction efficiency, good peak shape, and good separation effect

Active Publication Date: 2022-07-01
GUANGXI NANNING BAIHUI PHARMA GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, in the construction method of the HPLC fingerprint of the traditional Chinese medicine preparation, the determination of the content of a certain significant component is often used, and the efficacy of the traditional Chinese medicine preparation is often the result of the joint action of multiple active components. Therefore, the content of a certain component Qualified cannot represent the overall quality level and has great limitations
The Chinese invention patent with the notification number CN103926351B discloses a quality detection method for Shengxuebao preparations and a method for constructing standard fingerprints. Shengxuebao was tested for quality, with paeoniflorin as the reference substance, and 7 characteristic peaks were respectively identified to achieve the purpose of detecting the contents of 7 kinds of medicines in the prescription. The exclusive characteristic peak of Astragalus membranaceus, the other three medicines correspond to the common peak, and the method of constructing the map is complicated, which is not suitable for traditional Chinese medicine preparations with multiple prescriptions

Method used

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  • A kind of construction method of hplc fingerprint of germinal tablets
  • A kind of construction method of hplc fingerprint of germinal tablets
  • A kind of construction method of hplc fingerprint of germinal tablets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Construction of HPLC Fingerprint of Shengfa Tablets

[0042] 1. Preparation of the test solution: take the hair growth tablets, remove the coating, grind to get the test powder, take 0.5g of the test powder, accurately weigh it, and place it in a conical flask with a stopper. Add 10mL of solvent, weigh it, ultrasonically treat it (power 300W, frequency 40kHz) for 30 minutes, let it cool, weigh it again, use the solvent to make up the lost weight, shake well, pass through a 0.45μm filter membrane, and take the subsequent filtrate, namely have to;

[0043] 2. Preparation of reference substance solution: Weigh appropriate amount of stilbene glycoside, daidzein, terugenin, and paeoniflorin respectively, put them in the same volumetric flask, and add solvent to prepare a mixture containing 50 μg of each of the above reference substances per mL. reference solution;

[0044] 3. Preparation of medicinal material test solution: take 20 batches of finished products and referenc...

Embodiment 2

[0095] Construction of HPLC Fingerprint of Shengfa Tablets

[0096] 1. Preparation of the test solution: take this product, remove the coating, grind it finely to obtain the test powder, take 0.5g of the test powder, accurately weigh it, and place it in a conical flask with a stopper. Add 9 mL of solvent, weigh it, ultrasonically treat it (power 250W, frequency 35kHz) for 29 minutes, let it cool, weigh it again, supplement the lost weight with solvent, shake well, pass through a 0.45μm filter membrane, and take the subsequent filtrate, namely have to;

[0097] 2. Preparation of reference substance solution: Weigh appropriate amount of stilbene glycoside, daidzein, tegutrinoside, and paeoniflorin respectively, put them in the same volumetric flask, and add solvent to prepare a mixture containing 52 μg of each of the above reference substances per mL. reference solution;

[0098] 3. Preparation of medicinal material test solution: take 20 batches of finished products and refer...

Embodiment 3

[0104] Construction of HPLC Fingerprint of Shengfa Tablets

[0105] 1. Preparation of the test solution: take this product, remove the coating, grind it finely to obtain the test powder, take 0.5g of the test powder, accurately weigh it, and place it in a conical flask with a stopper. Add 9.5mL of solvent, weigh it, ultrasonically treat it (power 270W, frequency 36kHz) for 28 minutes, let it cool, weigh it again, use the solvent to make up the lost weight, shake well, pass through a 0.45μm filter membrane, and take the subsequent filtrate, that is obtained;

[0106] 2. Preparation of reference substance solution: Weigh appropriate amount of stilbene glycoside, daidzein, teruitrin, and paeoniflorin respectively, put them in the same volumetric flask, and add solvent to make a mixture containing 55 μg of each of the above reference substances per mL. reference solution.

[0107] 3. Preparation of medicinal material test solution: take 20 batches of finished products and refere...

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Abstract

The invention discloses a method for constructing an HPLC fingerprint of Shengfa tablets, belonging to the technical field of traditional Chinese medicine analysis. The traditional Chinese medicine tablets of Shengfa Tablets made from black dates and black beans were the detection targets, and high performance liquid chromatography was used to obtain the fingerprints of Shengfa Tablets by high performance liquid chromatography. The fingerprint spectrum constructed by the present invention has 10 common characteristic peaks, and 10 chemical components are confirmed and identified by using DAD detector and UFLC-TRIPLETOF-DAD-MS / MS technical means. The quality control method of the invention can comprehensively and systematically detect the medicinal materials in the prescription, and has practical application value for the quality control of the whole process of the preparation production process and the quality evaluation of the final product.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine analysis, and in particular relates to a method for constructing an HPLC fingerprint of a hair growth tablet prepared from traditional Chinese medicine raw materials. Background technique [0002] The hair growth tablet used in the present invention is a traditional Chinese medicine tablet made of Chinese medicine Polygonum multiflorum, Ligustrum lucidum, Eclipta chinensis, Rehmannia glutinosa, Chinese yam, Poria, Cortex Moutan, Alisma, mulberry, Ophiopogon japonicus, black jujube and black bean. Liver and kidney, nourishing qi and nourishing blood, hair growth and black hair function. It is used for premature graying and shedding of hair caused by deficiency of liver and kidney and deficiency of qi and blood; alopecia areata, alopecia totalis, and seborrheic alopecia, and has broad clinical application prospects. The invention provides a method for constructing the HPLC fing...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8634G01N30/8686
Inventor 姚宏亮刘颖彭维周雪芸王永刚李硕俊苏薇薇黄丽巧
Owner GUANGXI NANNING BAIHUI PHARMA GRP
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