Separation method of peripheral blood mononuclear cells

A separation method and peripheral blood technology, applied in the field of cell separation, can solve the problems of differences in purity and viability, influence of cell number and purity, and reduction of separation effect, and achieve the effect of high cell viability and purity.

Pending Publication Date: 2021-01-22
贵州北科生物科技有限公司
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Problems solved by technology

The purity of the cells obtained by this method is relatively good, but there are certain restrictions on the sample volume. If the sample volume exceeds 50ml, the separation effect will be greatly reduced; and the operation requirements are relatively high. If the operation is not careful enough, the separation The obtained cells will have a great influence on the quantity and purity
In addition, different centrifugal force and centrifugation time also have a great influence on the effect of cell separation. The cells separated by different centrifugation parameters have great differences in purity and viability.

Method used

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  • Separation method of peripheral blood mononuclear cells

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Embodiment Construction

[0040] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0041] It should be noted that all directional indications (such as up, down, left, right, front, back...) in the embodiments of the present invention are only used to explain the relationship between the components in a certain posture (as shown in the accompanying drawings). Relative positional relationship, movement conditions, etc., if the specific posture changes, the directional indication will also change accordingly.

[0042] In addition, the ...

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Abstract

The invention discloses a separation method of peripheral blood mononuclear cells. According to characteristics of separating a large number of blood samples, the method comprises steps of removing most of red blood cells by using a hydroxyethyl starch precipitation method, conducting natural sedimentation in the air, and further purifying the mononuclear cells by using a Ficoll density gradient centrifugation method to obtain PBMC with more than 95% of purity and more than 90% of activity. Compared with methods without natural sedimentation in the air, the cells obtained by the separation method of the present invention are higher in activity and purity.

Description

technical field [0001] The invention relates to the technical field of cell separation, in particular to a method for separating peripheral blood mononuclear cells. Background technique [0002] Cell therapy is a new technology for disease treatment that has emerged in recent years. It refers to the use of the characteristics of certain cells with specific functions, using bioengineering methods to obtain and or through in vitro expansion, special culture, etc., to make these cells have enhanced immunity. , killing pathogens and tumor cells, promoting the regeneration of tissues and organs and the recovery of the body, so as to achieve the purpose of treating diseases. [0003] With its good curative effect, less side effects, more individualized and individualized unique advantages, cell therapy provides a choice, sometimes even the last choice, for the treatment of some refractory diseases. Cell therapy will play an important role in clinical treatment both at present and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078
CPCC12N5/0634C12N2509/00C12N2509/10
Inventor 刘伟郑大学丁星
Owner 贵州北科生物科技有限公司
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