Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of indocyanine green nano medicine and preparation method thereof

A technology of indocyanine green and nano-drugs, which is applied in the field of biomedicine, can solve the problems of drug carrier oxidative damage, and achieve the effects of tumor targeting, improving blood circulation time, and strong hydrophilicity

Active Publication Date: 2021-11-16
YANSHAN UNIV
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have shown that PEG-modified drug carriers are prone to oxidative damage, and the body is prone to produce PEG antibodies after long-term use

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of indocyanine green nano medicine and preparation method thereof
  • A kind of indocyanine green nano medicine and preparation method thereof
  • A kind of indocyanine green nano medicine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] This example provides three kinds of indocyanine green nano-medicines, respectively G5MEKC(n)-ICG (n=20, 70 and 128), wherein the surface modification of the fifth generation polyamide-amine dendrimers (G5 PAMAM) The zwitterionic peptide is (EK) 3 -C polypeptide, its specific structure is as follows:

[0051]

[0052] (Glu-Lys) 3 -Cys polypeptide

[0053] Its preparation method comprises the following steps:

[0054] (1) The molar ratio of G5 PAMAM to maleic anhydride is 1:256; 8.0mg G5 PAMAM is dissolved in 1.5mL dimethyl sulfoxide solution, 6.9mg maleic anhydride is dissolved in 2mL dimethyl sulfoxide solution, The two were mixed well, and the reaction was stirred at room temperature for 24 hours. Then use a dialysis bag with a molecular weight cut-off of 8000-14000Da to dialyze in 2L of water for 6-8 hours to obtain the fifth-generation polyamide-amine dendrimer G5M aqueous solution containing double bonds on the surface;

[0055] (2) Press G5M and (EK) 3 -C...

Embodiment 2

[0067] This embodiment provides a kind of indocyanine green nanomedicine G5MDKC(10)-ICG, wherein the zwitterionic polypeptide modified on the surface of the fifth generation polyamide-amine dendrimer (G5 PAMAM) is (DK) 6 -C polypeptide, its specific structure is as follows:

[0068]

[0069] (Asp-Lys) 6 -Cys polypeptide

[0070] Its preparation method comprises the following steps:

[0071] (1) The molar ratio of G5 PAMAM to maleic anhydride is 1:640; 8.0mg G5 PAMAM is dissolved in 1.5mL dimethyl sulfoxide solution, 17.3mg maleic anhydride is dissolved in 2mL dimethyl sulfoxide solution, The two were mixed well, and the reaction was stirred at room temperature for 24 hours. Afterwards, a dialysis bag with a molecular weight cut-off of 8000-14000Da was dialyzed in 2L of water for 8 hours to obtain a fifth-generation polyamide-amine dendrimer G5M aqueous solution containing double bonds on the surface;

[0072] (2) Press G5M and (DK) 6 -C zwitterionic polypeptide molar r...

Embodiment 3

[0076] This embodiment provides a kind of indocyanine green nano drug G5MDQC(20)-ICG, wherein the zwitterionic polypeptide modified on the surface of the fifth generation polyamide-amine dendrimer (G5 PAMAM) is (DQ) 10 -C polypeptide, its specific structure is as follows:

[0077]

[0078] (Asp-Gln) 10 -Cys polypeptide

[0079] Its preparation method comprises the following steps:

[0080] (1) The molar ratio of G5 PAMAM to maleic anhydride is 1:384; 8.0mg G5 PAMAM is dissolved in 1.5mL dimethyl sulfoxide solution, 10.4mg maleic anhydride is dissolved in 2mL dimethyl sulfoxide solution, The two were mixed well, and the reaction was stirred at room temperature for 24 hours. Then use a dialysis bag with a molecular weight cut-off of 8000-14000Da to dialyze in 2L of water for 6-8 hours to obtain the fifth-generation polyamide-amine dendrimer G5M aqueous solution containing double bonds on the surface;

[0081] (2) Press G5M and (DQ) 10 -C zwitterionic polypeptide molar ra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides an indocyanine green nano-medicine and a preparation method thereof, belonging to the field of biomedicine. The indocyanine green nanomedicine comprises indocyanine green and a modified dendrimer carrier, the indocyanine green is coated on the inner cavity of the modified dendrimer carrier, and the modified dendrimer carrier has a general formula (I) Structure. The nanomedicine not only has the advantages of excellent anti-protein adsorption and high biocompatibility, but also has good photothermal tumor inhibition and near-infrared imaging effects.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an indocyanine green nano-medicine and a preparation method thereof. Background technique [0002] In recent years, cancer has become one of the major diseases that seriously affect human health and threaten human life. Most drug delivery systems, such as liposomes, dendrimers, and polymeric micelles, are widely used in cancer therapy. Due to the unique dendritic structure and the precise number of functional groups of polyamide-amine dendrimers, it has very good application prospects. As stable single-molecule structures, dendrimers can avoid structural collapse in physiological environments. In addition, by modifying targeting ligands, dendrimers can also achieve targeted delivery of drugs. The unique dendritic structure and abundant peripheral functional groups enable it to encapsulate small anti-cancer molecules in the cavity or modify the surface. However, the abundant amino g...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/62A61K47/69A61K49/00A61P35/00B82Y5/00
CPCA61K41/0052A61K49/0034A61K49/0054A61K49/0056A61K47/62A61K47/6949A61P35/00B82Y5/00
Inventor 王龙刚崔天明何蒙蒙张洁
Owner YANSHAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com