Immune cell line with enhanced anti-tumor effect
An immune cell, tumor technology, applied in the field of tumor cell immunotherapy, can solve problems such as limitation of anti-tumor activity and T cell apoptosis
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[0032] The method for constructing and verifying the cell line of the present invention comprises the following steps: step S1, site selection of RNAi
[0033] source of selection and lentiviral vectors;
[0034] Step S2, cell preparation;
[0035] Step S3, determining the drug sieve concentration;
[0036] Step S4, infection experiment of U937 cells; Step S5, drug screening;
[0037] Step S6, the expression identification of PrP;
[0038] Step S7, determination of the inhibitory effect of the cell line on tumor cells and the proliferation rate. Wherein, obtaining the construct of the cell line
[0039]Building further includes the following steps:
[0040] 1. Determine the interference sequence of the PRNP gene: GAGCAGGCCCATCATACAT. After the lentiviral vector is produced by a commercial company, the cell line is constructed according to the conventional experimental protocol.
[0041] 2. Cell preparation
[0042] (1) Resuscitating cells: Quickly take out the frozen ce...
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