An immune cell line with enhanced antitumor effect

A technology of immune cells and tumor cells, which is applied in the field of enhancing the anti-tumor effect of immune cells, and can solve the problems of T cell apoptosis and limitation of anti-tumor activity.

A technology of immune cells and tumor cells, which is applied in the field of enhancing the anti-tumor effect of immune cells, and can solve the problems of T cell apoptosis and limitation of anti-tumor activity.

CN112442485BActive Publication Date: 2022-07-05EAST CHINA NORMAL UNIV
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  • An immune cell line with enhanced antitumor effect
  • An immune cell line with enhanced antitumor effect
  • An immune cell line with enhanced antitumor effect

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The method for constructing and verifying the cell line of the present invention includes the following steps: Step S1, RNAi site selection

[0033] selection and source of lentiviral vectors;

[0034] Step S2, cell preparation;

[0035] Step S3, determine the drug sieve concentration;

[0036] Step S4, infection experiment of U937 cells; Step S5, drug screening;

[0037] Step S6, the expression identification of PrP;

[0038] Step S7, determination of the inhibitory effect of the cell line on tumor cells and the proliferation rate. wherein the structure of the cell line is obtained

[0039]The build further includes the following steps:

[0040] 1. Determine the interference sequence of the PRNP gene: GAGCAGGCCCATCATACAT. After the lentiviral vector is produced by a commercial company, the cell line is constructed according to the conventional experimental plan.

[0041] 2. Preparation of cells

[0042] (1) Resuscitate cells: Quickly take out the frozen cells fr...

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Abstract

The invention relates to the field of cellular immunotherapy of tumors, in particular to enhancing the anti-tumor effect of immune cells by modifying specific genes of immune cells, specifically reducing the expression of PRNP gene (prion protein, PrP, prion protein) by technical means to Enhance the anti-tumor effect of immune cells. By knocking down PrP in human histiocytic lymphoma U937 cell line, an immune cell line with low PrP expression was obtained. The cell line has an enhanced ability to inhibit the growth of human or mouse colon cancer and melanoma cells, and the immune cell line is induced by tumor cells to increase the level of self-cell growth.

Description

technical field [0001] The invention relates to the field of cellular immunotherapy of tumors, in particular to enhancing the anti-tumor effect of immune cells by modifying specific genes of immune cells, specifically reducing the expression of PRNP gene by technical means to enhance the anti-tumor effect of immune cells. Background technique [0002] Cancer is one of the major diseases that threaten human health today, and its treatment methods have attracted much attention. Among all tumor treatment methods, the efficacy of immune cell therapy has been clinically proven, and among them, Chimeric Antigen Receptor T-Cell Immunotherapy (CAR-T) is considered to be the most effective One of the promising treatments [1] . [0003] The basic principle of CAR-T action is to use immune cells to eliminate cancer cells. In a normal body, T cells can recognize abnormal cells by contacting MHC on the surface of abnormal cells through antigen-presenting cells to clear them; on the ot...

Claims

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Application Information

Patent Timeline
05 Jul 2022
Publication
CN112442485B
IPC
C12N5/10; A61K35/13; A61K35/17; A61P35/00
CPC
C12N5/0635; C12N5/0636; C07K14/47; A61K35/13; A61K35/17; A61P35/00
Inventors
赵宇岚; 张显凯