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A kind of veterinary vaccine nanoemulsion and preparation method thereof

A nanoemulsion and vaccine technology, applied in veterinary vaccines, biochemical equipment and methods, vaccines, etc., can solve the problems of coarse particle size, difficult to reach the size of fine milk, uneven distribution, etc.

Active Publication Date: 2022-07-05
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the particle size of the nanoemulsion prepared by the invention is within 200-500nm, the particle size is relatively coarse, and the distribution is uneven, and it is difficult to reach the fine emulsion scale (<100 nm) of the nano drug targeted delivery system

Method used

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  • A kind of veterinary vaccine nanoemulsion and preparation method thereof
  • A kind of veterinary vaccine nanoemulsion and preparation method thereof
  • A kind of veterinary vaccine nanoemulsion and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Preparation of avian influenza H7N9 inactivated vaccine nanoemulsion 1:

[0061] s1. Prepare the inner aqueous phase; disperse levamisole (mass fraction 1%) in the antigen solution, mix uniformly with 800 rpm magnetic stirring for 5 minutes, adjust the pH value to 6.9, prepare the antigen dispersion, and take 5 g as the inner aqueous phase;

[0062] s2, prepare the oil phase of the middle layer; stir and mix propylene glycol monolaurate and polyoxyethylene monolaurate in a ratio of 3:1 to obtain mixed surfactant one; then mix mineral oil, squalane and Mixing the surfactant 1 according to the ratio of 5:1:3, 800rpm magnetic stirring for 5min and mixing evenly to obtain the intermediate layer oil phase.

[0063] s3. Take 25gs2 of the oil phase of the intermediate layer and quickly pour it into the inner water phase of 5gs1, emulsify for 5min at a temperature of 25°C and a shear rate of 8000rpm, and stand for 10-20min to obtain water-in-oil colostrum.

[0064] ...

Embodiment 2

[0066] Example 2: Preparation of avian influenza H7N9 inactivated vaccine nanoemulsion 2:

[0067] s1. Prepare the inner water phase; disperse levamisole (mass fraction 1%) in the antigen solution, mix uniformly with 800 rpm magnetic stirring for 5 minutes, adjust the pH value to 6.9, prepare the antigen dispersion liquid, and take 10 g as the inner water phase;

[0068] s2, prepare the oil phase of the middle layer; stir and mix Span 85 and polyethylene glycol 15-hydroxystearate in a ratio of 2.5:1 to obtain mixed surfactant one, mix mineral oil, squalane and mixed Surfactant one is uniformly mixed by magnetic stirring at 720 rpm for 5 minutes at a ratio of 4:1:2 to obtain an oil phase in the middle layer;

[0069] s3, quickly pour the oil phase of the middle layer of 20gs2 into the inner water phase of 10gs1, and emulsify for 5min at a temperature of 25°C and a shear rate of 8000rpm to obtain water-in-oil colostrum;

[0070] s4. Prepare the external aqueous phase; take 7 g ...

Embodiment 3

[0072] Example 3: Preparation of avian influenza H7N9 inactivated vaccine nanoemulsion 3:

[0073] s1. Prepare the inner aqueous phase; disperse levamisole (mass fraction 1%) in the antigen solution, mix evenly with 800 rpm magnetic stirring for 5 min, adjust the pH value to 6.9, prepare the antigen dispersion, and take 15 g as the inner aqueous phase;

[0074] s2, prepare the oil phase of the middle layer; stir and mix glycerol monostearate and isopropyl myristate uniformly according to the ratio of 4:1 to prepare mixed surfactant one, mix mineral oil, squalane and mixed surface Active agent one is stirred and mixed uniformly according to the ratio of 5:2:2 to obtain the oil phase of the middle layer;

[0075] s3, quickly pour the oil phase of the middle layer of 15gs2 into the inner water phase of 15gs1, at a temperature of 25°C, a shear rate of 8000 rpm, and emulsification for 5min to obtain water-in-oil colostrum;

[0076] s4. Prepare the outer aqueous phase; take 7 g of ...

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Abstract

The invention discloses a veterinary vaccine nanoemulsion and a preparation method thereof. wherein the weight percentages of the above components are: 5% to 30% of the inner water phase, 20% to 50% of the oil phase in the middle layer, and 50% to 85% of the outer water phase; the preparation method of the veterinary vaccine nanoemulsion in the present invention is simple , cost saving; the present invention improves the stability and safety of the vaccine nanoemulsion through the layer-by-layer release of the antigen and / or immune enhancer; reduces the dispersion of the drug through the mutual cooperation between the inner water phase and the outer water phase and the oil in the middle layer to improve drug absorption.

Description

technical field [0001] The invention belongs to the technical field of veterinary medicine, in particular to a veterinary vaccine nanoemulsion and a preparation method thereof. Background technique [0002] The veterinary inactivated vaccine in my country is mainly composed of EC system, that is, it is mainly composed of injection oil, surfactant and antigen solution. At present, most of the emulsion vaccines have a particle size of more than 1 micron, which belongs to ordinary milk or coarse milk. Such emulsions are thermodynamically unstable systems, which are prone to delamination, flocculation, etc., resulting in demulsification and poor stability. In addition, the particle size of this type of emulsion is large, and the oil phase and the antigenic water phase are not fully dispersed, resulting in high viscosity, difficulty in injection, and serious side reactions at the injection site. At the same time, the antigen molecules cannot be fully absorbed by the animal body...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/113A61K9/107A61K47/06A61K39/39A61K39/395A61K39/145A61K39/135A61K39/17A61K39/225A61K39/215A61K39/275A61P37/04A61P31/16A61P31/14A61P31/20
CPCA61K9/113A61K9/1075A61K47/06A61K39/39A61K39/395A61K39/12A61P37/04A61P31/16A61P31/14A61P31/20A61K2039/552A61K2039/545A61K2039/5252C12N2760/16134C12N2770/32134C12N2760/18134C12N2770/20034C12N2710/24234A61K2300/00Y02A50/30
Inventor 崔海信郭亮赵翔王春鑫崔博
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI