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Preparation method of aptamer modified gold nanostars

A nucleic acid aptamer, gold nanotechnology, applied in the directions of nanotechnology, nanotechnology, metal processing equipment, etc., can solve the problems of difficulty in generating strong Raman signals, small Raman scattering cross-sections, and complex processes for detection objects, and achieve The effect of high Raman scattering enhancement factor, less use of reaction chemicals, and simple incubation steps

Pending Publication Date: 2021-04-13
杭州苏铂科技有限公司
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Problems solved by technology

[0007] In view of the small Raman scattering cross-section and low sensitivity when the existing Raman spectroscopy is used as an analysis method, the process of using noble metal nanoparticles to form "hot spots" in surface-enhanced Raman scattering is complex and difficult, and the detection objects are difficult to access and Enrichment in the "hot spots" of gold nanostars makes it difficult to generate strong Raman signals. The present invention provides a method for preparing gold nanostars modified by nucleic acid aptamers

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  • Preparation method of aptamer modified gold nanostars
  • Preparation method of aptamer modified gold nanostars
  • Preparation method of aptamer modified gold nanostars

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[0043] In the following description, the present invention is described with reference to various examples. One skilled in the art will recognize, however, that the various embodiments may be practiced without one or more of the specific details, or with other alternative and / or additional methods, materials, or components. In other instances, well-known structures, materials, or operations are not shown or described in detail so as not to obscure aspects of the various embodiments of the invention. Similarly, for purposes of explanation, specific quantities, materials and configurations are set forth in order to provide a thorough understanding of embodiments of the invention. However, the invention may be practiced without these specific details. Furthermore, it should be understood that the various embodiments shown in the drawings are illustrative representations and are not necessarily drawn to scale.

[0044]In this specification, reference to "one embodiment" or "the ...

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Abstract

The invention discloses a preparation method of aptamer modified gold nanostars. The preparation method comprises the steps of: preparing anisotropic gold nanostar (GNSt) solution; adding an aptamer into the anisotropic gold nanostar (GNSt) solution, and carrying out swirling; adding citrate-HCl buffer solution; adding NaCl solution, and after carrying out swirling, and standing for a certain time; after centrifuging the solution, re-dispersing the centrifuged solution in ultrapure water to obtain the required aptamer modified gold nanostar solution; and storing the aptamer modified gold nanostar solution for later use.

Description

technical field [0001] The invention belongs to the technical field of nano-biological materials, and relates to a preparation method of simple nucleic acid aptamer-modified gold nano-stars. Background technique [0002] Raman spectroscopy can be used as an analysis method to obtain the fingerprint of analyte molecules, but the Raman spectroscopy is limited in its application scenarios due to the small Raman scattering cross-section and low sensitivity. [0003] Surface-enhanced Raman scattering (SERS) can significantly amplify the Raman signal by several orders of magnitude, thereby improving the sensitivity of Raman detection. In the prior art, noble metal nanoparticles (NPs) are used as the active substrate material for surface-enhanced Raman spectroscopy (SERS), when the analyte molecules are located at the junctions between the nanoparticles, called "hot spots", this situation The Raman signal of the lower analyte is greatly enhanced. But the process of forming "hot s...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B22F1/00G01N21/65B82Y15/00B82Y30/00B82Y40/00
CPCB22F9/24G01N21/658B82Y15/00B82Y30/00B82Y40/00B22F1/0553B22F1/07B22F1/054B22F1/148
Inventor 周宇托尼·卡斯丹尼尔·埃尔森徐建华周玄杨光
Owner 杭州苏铂科技有限公司
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