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Method for quantitatively detecting saponin compounds

A quantitative detection and compound technology, applied in the field of analytical chemistry, can solve the problems such as difficulty in quality control of cotton wool, the detection method of saponin compounds needs to be studied, etc., and achieves the effect of avoiding thermal hydrolysis, high accuracy and sensitivity

Inactive Publication Date: 2021-04-13
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the 2015 edition of the "Chinese Pharmacopoeia" does not contain the content determination of the cotton scorpion, which brings some difficulties to the quality control of the scorpion.
Therefore, the detection method of saponins remains to be studied

Method used

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  • Method for quantitatively detecting saponin compounds
  • Method for quantitatively detecting saponin compounds
  • Method for quantitatively detecting saponin compounds

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Embodiment 1

[0040] In this example, the method for quantitatively detecting saponin compounds in the embodiment of the present invention is used to detect saponin compounds in Scorpio spp., specifically as follows:

[0041] 1. Experimental part

[0042] 1.1 Instruments and reagents_

[0043] The models and manufacturers of instruments and reagents are shown in Table 1. _

[0044] Table instrument and reagent information summary table

[0045]

[0046] 1.2 Preparation of standard solution_

[0047] Dioscin, protodioscin, and pseudoprotodioscin were purchased from ChromaDex Company in the United States; diosgenin and protodioscin were purchased from J&K Company; dioscin A, pseudoprotodioscin and trillium were purchased from Shanghai Yansheng Company , scutellaria saponin, triangularin and prototrianginin were purchased from Nanjing Senbega Company, and the mass fractions of 11 standard products were all ≥98%.

[0048] 11 kinds of saponin standard stock solutions: Accurately weigh 0....

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Abstract

The invention discloses a method for quantitatively detecting saponin compounds. According to the embodiment of the invention, the method comprises the following steps: crushing a sample to obtain a sample powder; carrying out ultrasonic extraction on the sample powder to obtain an extract; and detecting the extract by using a chromatographic-quadrupole / electrostatic field orbitrap high-resolution mass spectrometry system so as to obtain the content of the saponin compounds. According to the method, ultrasonic extraction is adopted, and saponin is effectively prevented from being hydrolyzed by heat. The chromatographic quadrupole / electrostatic field orbitrap high-resolution mass spectrometry system is adopted for detection, and the detection accuracy and sensitivity are high.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for quantitatively detecting saponin compounds. Background technique [0002] The main active ingredients in Mianzia are steroidal saponins, which have been mainly used as important raw materials for the synthesis of hormone drugs and contraceptives. In recent years, more and more studies have shown that steroidal saponins have a wide range of pharmacological effects and important biological activities, such as anti-tumor effect, hypoglycemic, anti-inflammatory, prevention and treatment of cardiovascular diseases, etc. Due to its remarkable curative effect in the treatment of cardiovascular and cerebrovascular diseases, there are many clinical applications, such as Di'ao Xinxuekang Capsules, which contain 8 kinds of saponins extracted from yellow yam and pangosaurus as the main components, and water-soluble saponins extracted from the rhizome of Dioscorea baicalensis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88G01N2030/884
Inventor 张峰冯峰
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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