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Wheat Root Length Molecular Markers and Its Application

A molecular marker, wheat technology, applied in biochemical equipment and methods, DNA/RNA fragments, microbial determination/inspection, etc.

Active Publication Date: 2022-06-14
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the identification process of soil root depth is complicated and tedious, while the identification of wheat root system by hydroponics is simple and convenient.

Method used

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  • Wheat Root Length Molecular Markers and Its Application
  • Wheat Root Length Molecular Markers and Its Application
  • Wheat Root Length Molecular Markers and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1. The discovery of the longest root length-related SNP markers in wheat and the development of KASP markers and the establishment of detection methods

[0051] 1. Identification of longest root length in wheat seedling stage

[0052] F 1 , F 1 F 2 , using the single-seed method to obtain F 7 Generation of recombinant inbred line (RIL) populations. Utilize hydroponic method to cultivate plant and measure its maximum root length, specific method is as follows:

[0053] Pick plump and consistent seeds 15 grains, 10% H 2 o 2 Sterilize and sterilize for 30 minutes, rinse with deionized water several times, place the seeds evenly on the culture tray covered with grids, the water surface in the culture tray just submerged the grids, and germinate at 23°C. Three days later, when the seed roots protrude into the water under the grid, move them into a light incubator (light / dark: 16h / 8h, temperature difference: 15°C / 20°C (light 20°C, dark 15°C)). After 7 days of ...

Embodiment 2

[0089] Example 2. Verification using the "Xiaoyan 54×Jing 411" recombinant inbred line population

[0090] F 1 , F 1 F 2 , using the single-seed method to obtain F 11 Generation "Xiaoyan 54×Jing 411" recombinant inbred line (RIL) population (XuYunfeng, An Diaoguo, Liu Dongcheng, Zhang Aimin, Xu Hongxing, Li Bin, Mapping QTL with epistatic effects and QTL 3treatment interactions for salt tolerance atseedling stage of wheat , Euphytica, 2012, 186(1):233-245.).

[0091] Measure the longest root length of the plant to be tested according to the method of step 1 in Example 1, extract the genomic DNA of each plant to be tested, and utilize the SNP typing method of step 3 in Example 1 to carry out genotyping of each strain .

[0092] The genotype and longest root length phenotype results of each plant to be tested are shown in Table 5, 6 and image 3 Shown: the genotype of the female parent Xiaoyan 54 (strain 1 in Table 5) is TT, and the longest root length is 12.75 cm; the gen...

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Abstract

The invention discloses wheat root length molecular markers and applications thereof. The wheat root length molecular marker disclosed by the present invention is a biallelic polymorphic SNP site, which corresponds to the nucleotide shown in the 156th position of SEQ ID No.1 in the sequence table in the wheat genome, and the wheat root length molecule Marked as C or T. Experiments have proved that the wheat root length molecular marker of the present invention is significantly correlated with the longest root length trait of the plant, indicating that the wheat root length molecular marker of the present invention and its set of primers can detect or assist in the detection of the longest root length trait of wheat.

Description

technical field [0001] The invention relates to the molecular marker of wheat root length and its application in the field of biotechnology. Background technique [0002] Wheat is one of the world's three major food crops, providing about 20% of human energy. In the 1960s, the "Green Revolution" marked by dwarf breeding transformed the aboveground traits of crops, enabling wheat and rice to have excellent characteristics such as high water and fertilizer resistance and lodging resistance, and thus revolutionaryly increased food production. . But while the "Green Revolution" increased food production, it also brought many problems. For example, high water and fertilizer planting methods lead to excessive consumption of water resources, eutrophication of water bodies, and land degradation. Even so, the current rate of increase in wheat varieties cannot meet the needs of the rapidly growing world population. People's research on crops and agricultural technology mainly focu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 郑琪罗巧玲杨国堂胡盼李宏伟李滨李振声
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI