A strain of avian influenza virus vaccine and its application

A bird flu virus and vaccine technology, applied in the direction of viruses, vaccines, antiviral agents, etc., can solve the problems of long disease duration and virus transmission of quail

Active Publication Date: 2022-02-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these viruses can cause disease in quail, infected quail have a longer course of disease than chickens and are therefore more likely to transmit the virus

Method used

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  • A strain of avian influenza virus vaccine and its application
  • A strain of avian influenza virus vaccine and its application
  • A strain of avian influenza virus vaccine and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Isolation and identification of quail-derived H9N2 subtype avian influenza virus GD / 07 / 17 strain

[0047] The H9 subtype avian influenza virus GD / 07 / 17 strain in the present invention is one of three low pathogenic avian influenza viruses isolated from quails in a certain area of ​​Guangdong Province, and has been identified by the Animal Influenza Laboratory of China Agricultural University , for the H9N2 subtype avian influenza virus. The three isolated strains are GD / 07 / 17, GD / 21 / 17 and GD / 11 / 18, named A / quail / Guangdong / 07 / 2017 (Qa / GD / 07 / 17), A / quail / Guangdong / 21 / 2017 (Qa / GD / 21 / 17) and A / quail / Guangdong / 11 / 2018 (Qa / GD / 11 / 18) strains, taxonomic name: Influenza A virus H9N2 subtype.

[0048] The isolation and identification process of the three strains is as follows:

[0049] (1) Processing of samples

[0050] To each swab sample, add 1ml of double antibody PBS solution containing (10000UL / ml), and mix by vortexing for 30S. The treated samples were stor...

Embodiment 2

[0075] Embodiment 2, the preparation of quail source H9N2 subtype AIV inactivated seedling

[0076] 1. Screening of vaccine virus strains

[0077] Test vaccine virus strains: (1) Three quail-derived H9N2 subtype AIVs, namely A / quail / Guangdong / 07 / 2017 (Qa / GD / 07 / 17), A / quail / Guangdong isolated in Example 1 / 21 / 2017 (Qa / GD / 21 / 17) and A / quail / Guangdong / 11 / 2018 (Qa / GD / 11 / 18). (2) Six existing quail-derived or chicken-derived H9N2 subtype AIVs preserved in our laboratory, namely A / chicken / Guangdong / 89 / 2017 (Ck / GD / 89 / 17), A / quail / Hongkong / G1 / 1997(Qa / G1 / 97), A / chicken / Beijing / F / 1998(Ck / F / 98), A / chicken / Shandong / ZB / 2007(Ck / ZB / 07), A / chicken / Jiangsu / TS / 2010 (Ck / TS / 10) and A / chicken / Beijing / 0701 / 2015 (Ck / BJ / 15).

[0078] The 10-fold diluted sera of the nine test strains were respectively diluted to a blood inhibitory titer of 1280 that reacted with self-antigens according to the corresponding times, and the isolated strains and antigenic analysis strains and antiserum were used for ...

Embodiment 3

[0099] Example 3. Evaluation of the protective properties of quail-derived H9N2 subtype AIV inactivated vaccines

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Abstract

The invention discloses an avian influenza virus vaccine strain and its application. The present invention provides H9N2 subtype quail-derived avian influenza virus A/quail/Guangdong/07/2017, and its preservation number in the General Microorganism Center of China Microbiological Culture Collection Management Committee is CGMCC No. 18509. The H9N2 subtype quail-derived avian influenza virus A/quail/Guangdong/07/2017 used in the present invention can be stably propagated in chicken embryos, the virus strain is stable, and has high safety, and is not pathogenic to quail; protective studies show that the vaccine It has a good protective effect on different branches of quail-derived H9N2 subtype AIV, can resist the infection of H9N2 virus with antigenic drift, and reduces the cost of research and development; the vaccine can also quickly (2 weeks after the first immunization) produce immune protection effect.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to an avian influenza virus vaccine strain and its application. Background technique [0002] At present, vaccination is the main means of preventing and controlling animal diseases in my country. The use of inactivated vaccines can be traced back to 1945, when whole virus inactivated vaccines were used in the United States and their clinical efficacy was observed. Because it retains all the antigenic components of influenza virus, it has good immunogenicity and safety, no mutation and virulence recovery, and the preparation process is simple, and the finished product is easy to store. It is widely used in the prevention of avian influenza pandemics. . [0003] At present, the commonly used influenza vaccine in quail is developed by chicken-derived H9N2 subtype AIV, and there is almost no recombinant inactivated vaccine developed for quail-derived H9N2 subty...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00C12N7/06A61K39/145A61P31/16C12R1/93
CPCC12N7/00A61K39/12A61P31/16C12N2760/16121C12N2760/16163A61K2039/552C12N2760/16034
Inventor 孙怡朋刘金华王贞
Owner CHINA AGRI UNIV
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