A strain of avian influenza virus vaccine and its application
A bird flu virus and vaccine technology, applied in the direction of viruses, vaccines, antiviral agents, etc., can solve the problems of long disease duration and virus transmission of quail
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Embodiment 1
[0046] Example 1. Isolation and identification of quail-derived H9N2 subtype avian influenza virus GD / 07 / 17 strain
[0047] The H9 subtype avian influenza virus GD / 07 / 17 strain in the present invention is one of three low pathogenic avian influenza viruses isolated from quails in a certain area of Guangdong Province, and has been identified by the Animal Influenza Laboratory of China Agricultural University , for the H9N2 subtype avian influenza virus. The three isolated strains are GD / 07 / 17, GD / 21 / 17 and GD / 11 / 18, named A / quail / Guangdong / 07 / 2017 (Qa / GD / 07 / 17), A / quail / Guangdong / 21 / 2017 (Qa / GD / 21 / 17) and A / quail / Guangdong / 11 / 2018 (Qa / GD / 11 / 18) strains, taxonomic name: Influenza A virus H9N2 subtype.
[0048] The isolation and identification process of the three strains is as follows:
[0049] (1) Processing of samples
[0050] To each swab sample, add 1ml of double antibody PBS solution containing (10000UL / ml), and mix by vortexing for 30S. The treated samples were stor...
Embodiment 2
[0075] Embodiment 2, the preparation of quail source H9N2 subtype AIV inactivated seedling
[0076] 1. Screening of vaccine virus strains
[0077] Test vaccine virus strains: (1) Three quail-derived H9N2 subtype AIVs, namely A / quail / Guangdong / 07 / 2017 (Qa / GD / 07 / 17), A / quail / Guangdong isolated in Example 1 / 21 / 2017 (Qa / GD / 21 / 17) and A / quail / Guangdong / 11 / 2018 (Qa / GD / 11 / 18). (2) Six existing quail-derived or chicken-derived H9N2 subtype AIVs preserved in our laboratory, namely A / chicken / Guangdong / 89 / 2017 (Ck / GD / 89 / 17), A / quail / Hongkong / G1 / 1997(Qa / G1 / 97), A / chicken / Beijing / F / 1998(Ck / F / 98), A / chicken / Shandong / ZB / 2007(Ck / ZB / 07), A / chicken / Jiangsu / TS / 2010 (Ck / TS / 10) and A / chicken / Beijing / 0701 / 2015 (Ck / BJ / 15).
[0078] The 10-fold diluted sera of the nine test strains were respectively diluted to a blood inhibitory titer of 1280 that reacted with self-antigens according to the corresponding times, and the isolated strains and antigenic analysis strains and antiserum were used for ...
Embodiment 3
[0099] Example 3. Evaluation of the protective properties of quail-derived H9N2 subtype AIV inactivated vaccines
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