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Hericium erinaceus full-nutrition fed-batch semi-continuous fermentation method

A nutrient flow, Hericium erinaceus technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of decreased polysaccharide content of bacteria powder, thick fermentation liquid, concentrated fermentation tank, etc. High yield, high polysaccharide content, and effect of shortening fermentation time

Active Publication Date: 2021-04-30
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fermentation of Hericium erinaceus generally has the problem of long fermentation time, thick fermentation liquid, and serious concentration in the fermentation tank; at the same time, if the fermentation time is too long, the polysaccharide content of the bacteria powder will also decrease.

Method used

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  • Hericium erinaceus full-nutrition fed-batch semi-continuous fermentation method
  • Hericium erinaceus full-nutrition fed-batch semi-continuous fermentation method
  • Hericium erinaceus full-nutrition fed-batch semi-continuous fermentation method

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A method for fully nutrient fed-batch semi-continuous fermentation of Hericium erinaceus, the concrete steps are as follows:

[0037](1) Seed tank culture: under aseptic conditions, Hericium erinaceus (strain preservation number is CGMCC 20754, preserved in the General Microbiology Center (CGMCC) of China Committee for the Collection of Microorganisms, address: Chaoyang District, Beijing No. 3, No. 1 Yard, Beichen West Road, Institute of Microbiology, Chinese Academy of Sciences; date of preservation: October 29, 2020) the slant strains were crushed and inoculated in the seed liquid medium, and the liquid medium for the seed tank consisted of 1.42% soybean meal without dregs , peptone 0.535%, glucose 3.03%, KH 2 PO 4 0.15%, MgSO 4 0.075% and 0.178% antifoaming agent, add water to make seed solution, the filling volume is 80%, the pH is 5.2, the temperature is 26.3°C, and the incubation time is 95h.

[0038] (2) Fermenter culture: inoculate 1% seed liquid into the f...

Embodiment 2

[0042] (1) Seed tank culture: under aseptic conditions, the slant strains of Hericium erinaceus were smashed and inoculated in a 30L fermenter with seed liquid medium. %, Glucose 3.03%, KH 2 PO 4 0.15%, MgSO 4 0.075%, antifoaming agent 0.178%, add water to 24L to make seed liquid, liquid volume 80%, pH 5.2, temperature 26.3 ℃, incubation time 95h.

[0043] (2) Fermentation tank culture: inoculate 1% seed liquid to the fermenter tank, the total volume of the fermentation medium is 80L, wherein 60% of the medium composition is used as the initial medium to fix the volume to 67L, and it is added to the 100L fermenter at one time, and the remaining Add 40% of the culture medium components to 13L as a supplementary liquid, feed it into the fermenter from 0h of fermentation, feed it throughout the fermentation process, and obtain the fermentation liquid; at the same time, observe the volume of the fermentation liquid in the tank every 2 hours, and take the excess fermentation li...

Embodiment 3

[0045] A method for Hericium erinaceus full-nutrition fed-batch semi-continuous fermentation, with reference to Example 1, the difference is that: (1) in the seed tank culture, the seed medium uses 1.6% non-residue soybean meal, the pH is 4.8, and the fermentation temperature is 26.0 ℃; (2) The fermentation medium in the fermenter culture uses 3.5% deslagging soybean meal, the total volume of the fermentation medium is 24L, and 70% of the ingredients are used as the initial medium to fix the volume to 20L, and are added to the 30L fermenter at one time. Dilute the medium to 4 L with 30% of the medium components as a supplement, the pH is 4.8, the fermentation temperature is 26.0° C., and the culture time is 120 h.

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Abstract

The invention discloses a hericium erinaceus full-nutrition fed-batch semi-continuous fermentation method. The method comprises seed tank culture and fermentation tank culture, and is characterized in that an adopted seed culture medium comprises 1.3-2.0% of soybean meal, 0.4-0.6% of peptone, 2.8-3.2% of glucose, 0.12-0.18% of KH2PO4, 0.07-0.08% of MgSO4 and 0.16-0.18% of a defoaming agent; wherein the fermentation medium is prepared from 2.8-3.5% of bean pulp, 0.8-1.2% of glucose, 2.8-3.2% of molasses, 1.8-2.2% of corn starch, 18-22mg / L of amylase and 0.1% of a defoaming agent, the bean pulp in the seed medium is residue-free bean pulp, and the bean pulp in the fermentation medium is residue-free bean pulp. According to the method, the growth speed of hericium erinaceus thalli is increased, the yield of hyphae is increased, the fermentation time is shortened, and the content of polysaccharide and polypeptide is high.

Description

technical field [0001] The invention relates to an improvement of a Hericium erinaceus fermentation medium and a technique for optimizing a fermentation method, and belongs to the field of Hericium erinaceus fermentation. Background technique [0002] Hericium erinaceus, also known as Hericium erinaceus, Hedgehog, Hericium erinaceus, etc., belongs to Basidiomycotina, Russulales, Hericiumaceae, and Hericium genus. "The good name. According to records, Hericium erinaceus is flat in taste and sweet in taste, and has functions such as helping digestion and benefiting the five internal organs. Hericium erinaceus contains a variety of chemical components such as sugars, terpenes, phenols, and steroids. Pharmacodynamic studies have shown that Hericium erinaceus has various pharmacological effects such as anti-inflammatory, anti-ulcer, anti-tumor, anti-aging, and lowering blood sugar. Wild Hericium grows slowly and has limited resources. The artificial cultivation period is long...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/38C12R1/645
CPCC12N1/14C12N1/38Y02P60/87
Inventor 徐庆阳袁峰王鹏王英燕范祎立冯杰
Owner JIANGSU SHENHUA PHARMA
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