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Composition for improving number and lethality of autologous lymphocytes and application of composition

A technology of lymphocytes and compositions, applied in the direction of cell culture active agents, animal cells, vertebrate cells, etc., which can solve the problems of restricting wide application and difficult quality control

Pending Publication Date: 2021-04-30
山东凌曼生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing in vitro induced and expanded autologous lymphocyte preparation technology cannot be industrialized and the quality is difficult to control, which limits its wide clinical application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] A composition for increasing the number and killing ability of autologous lymphocytes, including anti-human CD3 monoclonal antibody, interleukin, compound nutritional composition, autoplasma, heparin, colchicine, phytochemical PP (PHA-P), wherein Anti-human CD3 monoclonal antibody 150-250ng, interleukin 500-1500ng, compound nutritional composition 1500-2500Ug, serum 0.8-1.2mL, heparin 20-28Ug, phytochemical P500-1500ng.

[0017] Specifically, independently packaged colchicine is also included. Before the culture is terminated, colchicine at a concentration of 40 μg / mL is added to the above composition, and the added amount is a final concentration of 0.4-0.8 μg / mL.

[0018] Specifically, the compound nutritional composition includes amino acids, carbohydrates, inorganic salts, and vitamins, wherein the amino acids include 200 parts of arginine, 20 parts of aspartic acid, 50 parts of asparagine, 20 parts of glutamic acid, glutamine Amide 300 parts, glycine 10 parts, hist...

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Abstract

The invention relates to a composition for improving the number and lethality of autologous lymphocytes and application of the composition. The composition comprises an anti-human CD3 monoclonal antibody, interleukin, a composite nutrient composition, calf serum, heparin, colchicine and phytohemagglutinin P (PHA-P). The composition includes 150-250 ng of the anti-human CD3 monoclonal antibody, 500-1500 ng of the interleukin, 1500-2500 Ug of the composite nutrient composition, 0.8-1.2 mL of the serum, 20-28 Ug of the heparin and 500-1500 ng of the phytohemagglutinin. The culture solution prepared from the composition plays a positive role in production of typeface lymphocytes, and under the action of an anti-human CD3 monoclonal antibody, interleukin and phytohemagglutinin (PHA), the small lymphocytes can be stimulated to be converted into lymphoblasts and enter mitosis. After short-term culture, a large number of mitotic cells can be obtained through colchicine treatment, hypotonic treatment and immobilization, and quantitative production is facilitated.

Description

technical field [0001] The invention relates to the technical field of lymphocyte culture, in particular to a composition for increasing the number and killing ability of autologous lymphocytes and its application. Background technique [0002] Lymphocytes are a type of white blood cells and are the smallest white blood cells. It is produced by lymphatic organs and mainly exists in the lymph fluid circulating in the lymphatic vessels. It is an important cellular component of the body's immune response function, and is the main executor of almost all immune functions of the lymphatic system. Frontline "soldiers". Lymphocytes are a type of cell line with immune recognition function. According to their migration, surface molecules and functions, they can be divided into T lymphocytes (also known as T cells), B lymphocytes (also known as B cells) and natural killer cells. (NK) cells. Both T cells and B cells are antigen-specific lymphocytes, and their initial source is the sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0781C12N5/0783
CPCC12N5/0635C12N5/0636C12N2500/12C12N2500/16C12N2500/30C12N2500/32C12N2500/35C12N2500/38C12N2501/2302C12N2501/2307C12N2501/2315C12N2501/515C12N2501/91C12N2501/999
Inventor 孔祥龙王巍然张波
Owner 山东凌曼生物科技有限公司
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