Use of beta-carboline alkaloid GAK in preparation of products for treating and/or preventing pulmonary fibrosis

A technology of alkaloids and products, applied in the field of preparing products for treating and/or preventing pulmonary fibrosis by β-carboline alkaloid GAK

Inactive Publication Date: 2021-05-18
LINYI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Studies have shown that β-carboline alkaloid GAK has antioxidant activity. In addition, there is no application research on other biological activities of GAK, which needs to be further explored

Method used

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  • Use of beta-carboline alkaloid GAK in preparation of products for treating and/or preventing pulmonary fibrosis
  • Use of beta-carboline alkaloid GAK in preparation of products for treating and/or preventing pulmonary fibrosis
  • Use of beta-carboline alkaloid GAK in preparation of products for treating and/or preventing pulmonary fibrosis

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Embodiment 1

[0028] 1. Experimental method

[0029] (1) Experimental animals, establishment of PF mouse model and experimental grouping

[0030] Clean-grade healthy C57BL / 6 mice, 80, male and female, 8-10 weeks old, weighing 20-24g. The feeding environment is suitable, well-ventilated, quiet, 12-hour circadian rhythm is maintained, the temperature is 25±2°C, the humidity is 45%-60%, the sexes are separated, the litter is changed every 2 days, free drinking water, daily pellet feed feeding , free to eat and drink. After one week of acclimatization, the experimental animals were randomly divided into groups according to body weight for the experiment. All mice were starved for 12 hours before the experiment, and the experimental operations were performed between 10:00am and 4:00pm.

[0031] The mouse PF model was established by dripping bleomycin solution into the trachea. After the mouse was anesthetized and fixed with 10% chloral hydrate, a 1 cm incision was made along the midline of th...

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Abstract

The invention discloses use of a beta-carboline alkaloid GAK in preparation of products for treating and/or preventing pulmonary fibrosis. According to the use, the bioactivity of the beta-carboline alkaloid GAK in treatment on the pulmonary fibrosis is researched, and the condition that the beta-carboline alkaloid GAK has a relatively good improvement action on body weight, survival rate, pulmonary index and tissue pathological changes of PF mice and is dose-dependent is verified. A Masson staining and hydroxyproline assaying result shows that the GAK can be used for obviously inhibiting collagen deposition of pulmonary tissue; and an immunization fluorescence staining result shows that an epithelial cell marker protein E-Cadherin in the pulmonary tissue of mice of a GAK-dosed group is increased obviously, an interstitial cell marker protein alpha-SMA in the pulmonary tissue is decreased obviously, and thus, the GAK has an obvious inhibiting action on an EMT process of a fibrosis stage. The above results show that the GAK has anti-PF activity in an animal body, a theoretical foundation is provided for researching and developing natural product drugs for treating the pulmonary fibrosis, and the GAK is advantageously used for treating the pulmonary fibrosis.

Description

technical field [0001] The present invention relates to the medical application field of β-carboline alkaloid GAK, in particular to the use of β-carboline alkaloid GAK in preparing products for treating and / or preventing pulmonary fibrosis. Background technique [0002] Gansu flea, also known as Gansu snow ganoderma, is produced in southeastern Tibet, Qinghai, northwestern Sichuan, and western Gansu. It is born in alpine meadows and gravel flow belts at an altitude of 4,000 to 5,000 meters. It is a traditional Tibetan medicine. [0003] Traditional Tibetan medicine is of great significance to the development of natural product drugs. Gansu flea mainly contains a variety of active ingredients, such as β-carboline alkaloids, steroids, triterpenoids, flavonoids, amino acids, stearin acid, a-trehalose, etc. However, due to the lack of suitable chromatographic purification methods, there are few reports on the specific chemical components of Gansu flea, and the specific applicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/437A61P11/00
CPCA61K31/437A61P11/00
Inventor 崔玉磊沈娜辛化伟
Owner LINYI UNIVERSITY
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