A kind of biofilm carrier and domestication method thereof

Active Publication Date: 2021-09-14
山东省海洋资源与环境研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Shellfish purification of aquaculture sewage has the unique advantages of low price and wide sources. However, compared with the microbial population of activated sludge, shellfish only has a better filtering effect on large particles of organic suspended matter, and its range of action is narrower.

Method used

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  • A kind of biofilm carrier and domestication method thereof
  • A kind of biofilm carrier and domestication method thereof
  • A kind of biofilm carrier and domestication method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] This example provides a method for inoculating and culturing clams with four corners.

[0066] (1) Preparation of water body for inoculation:

[0067] Prepare a separate water storage tank with a volume of 50L, add 29L of seawater for culture and 1L of shrimp culture pond water and mix well, add 500mL of Nannochloropsis algae liquid, keep the water temperature at 17°C, and store for 5 days with dissolved oxygen at 7mg / L. Avoid direct sunlight during preparation.

[0068] (2) Target carrier temporary support

[0069] Brush the surface of the four-cornered clams transported back to the laboratory, select individuals with the same size and no damage, and temporarily raise them for 6 days. The volume of the temporarily raised water tank is 50L, add 30L of seawater, and 80 four-cornered clams. Keep the dissolved oxygen at 7 mg / L, and change the water once a day. If there is a dead body in the tank, immediately pick out the dead body and change the water. No bait was cast...

Embodiment 2

[0077] The difference between this example and Example 1 is that the shrimp culture pond water (1L) in step (1) was replaced with eutrophic nearshore seawater (1L), and the nearshore seawater was collected from the seaside of Yantai City.

[0078] Through 2 weeks of observation, during the first few days (4th day) of domestication, the number of sessile ciliates of Protozoan Ciliateta was relatively small, and the number of swimming ciliates was more. As the culture time progressed, when the micro organisms in the sludge gradually adapted to the new water quality and growth environment (7th day), sessile ciliates began to appear in large numbers, accompanied by a very small amount of swimming ciliates. At this time, the cultivation has been basically completed, and the inoculation is considered successful. At the late stage of culture (14th day), the sessile ciliates had been dominant. Microscopic examination was carried out on the gills and other tissues of the four-cornered...

Embodiment 3

[0081] The four-cornered clam successfully inoculated in Example 1 was used as the mother generation, and the uninoculated four-cornered clam was subcultured.

[0082] Add 30 liters of seawater to the water tank, put 40 successfully inoculated female four-cornered clams and 40 uninoculated four-cornered clams, keep the dissolved oxygen at 7 mg / L, feed 650 mL of trigonaria per day, and change the water every 24 hours. Eliminate dead individuals.

[0083] On the 7th day, the offspring four-horned clam was taken for microscopic examination, and it was found that bell worms appeared in the border (see Figure 7 ) and Cyclops (see Figure 8 ), and occasionally a small amount of polyshinites can be seen (see Figure 9 ) and weedworms (see Figure 10 ), considered successful inoculation. On the 14th day, the progeny of the four-cornered clam was taken for microscopic examination, and the sessile ciliates were absolutely dominant. Microscopic examination was carried out on the gi...

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Abstract

The invention discloses a biofilm carrier and a domestication method thereof, belonging to the field of environmental science. In the present invention, the four-cornered clam is cultivated in the inoculated water body until the microbiological colony grows stably at the edge of the four-cornered clam, and the four-cornered clam that can stably grow the micro-biological community on the edge is used as a biofilm carrier, and the micro-biological colony and the four-cornered clam are used The symbiotic relationship formed can effectively deal with eutrophication in aquaculture wastewater, purify sea water quality, and improve the survival rate of four-cornered clams in high-density applications.

Description

technical field [0001] The invention relates to the field of environmental science, in particular to a biofilm carrier and a domestication method thereof. Background technique [0002] The near-shore sea area is the area most closely related to human activities. It is not only affected by various natural forces on land and sea, but also deeply affected by human activities. In recent years, the environmental pollution problem in the near-shore sea area has become more and more serious. The proportion of pollution is increasing year by year. The reason for the pollution of aquaculture tail water is the rapid intensification and large-scale aquaculture mode of aquaculture industry in my country. In high-density farming activities, due to the lack of unified operating procedures, residual bait, feces and other residues generated during the breeding process will easily cause pollution to the seawater environment if they are discharged indiscriminately without treatment. It is e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/10C12N1/20C12N1/12A01K61/54
CPCA01K61/54C12N1/10C12N1/12C12N1/20Y02A40/81
Inventor 王玮云张秀珍秦华伟何金霞任利华刘丽娟刘慧慧黄会姜向阳程玲姜芳
Owner 山东省海洋资源与环境研究院
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