Heparin binding protein determination kit

Abstract

The invention discloses a heparin binding protein determination kit. The heparin binding protein determination kit comprises a first reagent, a second reagent and a test strip, the heparin binding protein determination kit can quantitatively and objectively reflect the existence of HBP, and provides a more powerful experimental diagnosis basis for clinical diagnosis, curative effect observation and prognosis judgment. The heparin binding protein determination kit provided by the invention is high in specificity, good in repeatability, high in detection sensitivity, wide in linear range and high in stability.

Description

Technical field[0001]The invention belongs to the field of biological detection, and more particularly to heparin binding protein assay kits, particularly involving an immunofluorescent chromatography kit and its application thereof.Background technique[0002]Heparin-BindingProtein (HBP) is also known as Azurocidin or CAP37, is a particulate protein of neutrophil, chemotactic activity, bactericidal capacity, and heparin binding ability with itself with strong positive charge with itself. Related to HBP as a chemokine, monocyte and macrophages, induced vascular leakage and tissue edema, in vitro energy induced Ca2 + dependent cytoskeletal rearrangement, and the formation of single-layer endothelial cell gaps. HBP can increase the permeability of vascular endothelial cells because HBP has strong positive charge, contacting endothelial cells, resulting in rapid activation of endothelial cells, forming actin tension fibers, resulting in leakage of cellular passage. The high concentration...

AI Technical Summary

Problems solved by technology

Although colloidal gold method and immunofluorescence chromatography can be quickly detected, they can only perform qualitative or semi-quantitative detection, with low accuracy and cannot provide accurate clinical judgment basis

At the same time, limited by the method itself, due to the use of a heterogeneous reaction system, the detection precision is poor, and the interference cannot be removed from the operating procedure, so that the accuracy of the test cannot be guaranteed