Kit for detecting human optimal partner potential genotype and method thereof

A partner and genotyping technology, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problem of reduced error avoidance ability of carriers

Inactive Publication Date: 2021-06-11
NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The gene rs1800497 (T / C) polymorphism can regulate the density of dopamine D2 receptors, and the T allele will lead to a decrease in the number of dopamine binding sites in the brain, making the carrier's ability to avoid errors reduced

Method used

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  • Kit for detecting human optimal partner potential genotype and method thereof
  • Kit for detecting human optimal partner potential genotype and method thereof
  • Kit for detecting human optimal partner potential genotype and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Selected sample: DNA / RNA-free water.

[0041] (2) System configuration

[0042] According to the instructions, prepare the primer Primer Mix and PCR reaction solution on ice according to the ratio of the instructions to configure the reaction system. After vortexing and mixing, centrifuge with a centrifuge, and then use a pipette tip to mix and distribute.

[0043] (3) Add samples

[0044] According to the instructions, use a pipette to take the corresponding volume of DNA / RNA-free water and add it to the reaction system that has been aliquoted.

[0045] (4) Amplification program

[0046] The amplification program on the PCR instrument is shown in Table 2.

[0047] (5) The amplified product is detected on the 3500DX genetic analyzer

[0048] The sample loading mixture is composed of deionized formamide and molecular weight internal standard (Size-500) in the system {(1μL Size-500+12μL deionized formamide)×(injection number)}. Mix 9 μL of the loading mixture wit...

Embodiment 2

[0054] (1) Selected samples: exfoliated cell samples from the oral cavity wall of subject 1 and subject 2.

[0055] (2) Sample collection

[0056] Collection type: Exfoliated cells from the inner wall of the oral cavity.

[0057] Collection method: Use the saliva collection stick to wipe back and forth on the inner wall of the oral cavity 4 times, continue to wipe back and forth on the inner wall of the oral cavity 4 times with the reverse side of the saliva collection stick, take out the saliva collection stick, and press repeatedly on the saliva sample collection card to collect the saliva inner wall cells Transfer to the saliva sample collection card, and dry the collected saliva sample collection card in a pollution-free area.

[0058] Valid sample: The area where the pink area of ​​the saliva sample collection card changes to light pink or white is the valid saliva sample area.

[0059] Sample selection method: use Dubbo plastic puncher (1.0mm) for manual punching and s...

Embodiment 3

[0079] (1) Selected sample: the blood sample of subject 2.

[0080] (2) Sample type: blood sample.

[0081] (3) DNA extraction: DNA extraction is performed on the blood sample using a nucleic acid extraction instrument.

[0082] (4) System configuration

[0083] According to the instructions, prepare the primer Primer Mix and PCR reaction solution on ice according to the ratio of the instructions to configure the reaction system. After vortexing and mixing, centrifuge with a centrifuge, and then use a pipette tip to mix and distribute.

[0084] (5) Adding samples: According to the instructions, take a certain amount of extracted DNA samples with a pipette and add them to the reaction system.

[0085] (6) Amplification program

[0086] The amplification program on the PCR instrument is shown in Table 2.

[0087] (7) The amplified product is detected on the 3500DX genetic analyzer

[0088] The sample loading mixture is composed of deionized formamide and molecular weight in...

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Abstract

The invention discloses a kit for detecting a human optimal partner potential genotype and a method thereof. The method adopts multiple PCR amplification and electrophoresis methods to analyze and identify allele polymorphisms (SNP) of seven genes: SNAP25, CLOCK, OXTR, DRD2, CADM2, FOXP2 and KATNAL2. The method comprises the following steps: a) collecting exfoliated cells in the oral cavity of a testee and storing the collected exfoliated cells in a collection card or performing DNA nucleic acid extraction on a blood sample; b) adding a saliva collection card sample or an extracted DNA sample of a testee into a reaction system, and carrying out PCR amplification; c) running an amplification program; and d) carrying out electrophoretic analysis on the amplification product, and interpreting according to a peak pattern graph. According to the invention, SNP of a plurality of genes related to a testee can be synchronously detected, and simplicity, high efficiency and specificity of detection are realized. Through the analysis, a reference is provided for whether the testee has the potential of the best partner or not.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a kit and method for detecting potential genotypes of human best partners. Background technique [0002] With the continuous development of scientific research in the field of molecular biology, more and more gene polymorphisms have been confirmed to be significantly correlated with diseases, language expression, responsiveness, intelligence, etc. These research results can be used for the evaluation of genetic predisposition in health, talent, personality, etc., and provide scientific and personalized guidance for the education and training of individual children. [0003] Cooperative ability is a natural ability, which is affected by genetic polymorphisms such as SNAP25, CLOCK, OXTR, DRD2, CADM2, FOXP2, and KATNAL2. Mainly manifested in non-verbal IQ, empathy, sense of responsibility, concentration, responsiveness, error avoidance, language control, emotional control, etc. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/166C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2545/101C12Q2565/125
Inventor 张成徐智孔咪咪余丁吴勇
Owner NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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