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Method for simultaneously determining contents of various antiepileptic drugs in blood and application of method

An anti-epileptic drug and content technology, applied in the field of medical detection, can solve the problems of complex pretreatment process, reduced processing capacity, undesired organic pollution, etc., and achieves the effect of specific detection limit and quantification limit, high sensitivity and reducing side effects.

Active Publication Date: 2021-06-11
JINAN YING SHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the sample pretreatment procedures including solid-phase extraction and liquid-liquid extraction are relatively complicated, for example, the organic layer after centrifugation is separated by gentle nitrogen flow and evaporated to dryness at 40 °C
This pretreatment not only increases the complexity of the operation and reduces the processing capacity, but also causes undesirable organic contamination, which is unacceptable for clinical laboratories

Method used

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  • Method for simultaneously determining contents of various antiepileptic drugs in blood and application of method
  • Method for simultaneously determining contents of various antiepileptic drugs in blood and application of method
  • Method for simultaneously determining contents of various antiepileptic drugs in blood and application of method

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Effect test

Embodiment 1

[0064] A method for simultaneously measuring the content of multiple antiepileptic drugs in plasma, comprising the steps of:

[0065] first step:

[0066] Preparation of reference substance solution: the stock solution of 9 kinds of antiepileptic drugs was dissolved in dimethyl sulfoxide with accurately weighed standard reference substance, and the final concentration of 9 kinds of antiepileptic drugs was 1000 μg / mL. Transfer 0.10 mL of precise volumes of standard solutions of nine antiepileptic drugs into a 10 mL volumetric flask, and dilute to volume with acetonitrile to obtain a working solution of nine antiepileptic drugs at 10 μg / mL. Dilute with acetonitrile to obtain working solutions of 2, 4, 10, 20, 100, 200, 400 and 600 ng / mL. At the same time, the isotopic internal standard raw materials of nine antiepileptic drugs were dissolved in a certain volume of dimethyl sulfoxide to prepare a 1000 μg / mL internal standard stock solution. The working solution concentration of...

Embodiment 2

[0091] A method for simultaneously measuring the content of multiple antiepileptic drugs in plasma and its application, comprising the following steps:

[0092] Pharmacokinetic studies were performed on 12 healthy male rats. The ethics committee approved the experimental protocol. Fast for 12 hours before administration and 2 hours after administration. After intragastric administration, 2 mL blood samples were drawn from the jugular vein before administration and at 0 o'clock. Blood was collected at 0.083, 0.167, 0.5, 0.75, 1, 2, 5, 8, 12 and 24 hours afterward. During the experiment, water was available freely. Plasma was then prepared by centrifugation at 14000 xg for 10 minutes and immediately frozen at -80°C.

[0093] Put 50 μL of rat plasma in a 2.0 mL centrifuge tube, add 200 μL of precipitated protein solvent containing 9 kinds of antiepileptic drug internal standards, shake for 10 min to extract analytes and internal standards, and centrifuge at 14,000 rpm for 15 ...

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Abstract

The invention provides a method for simultaneously determining the content of various antiepileptic drugs in blood and application of the method, and belongs to the technical field of medicine detection. According to the method of the invention, a protein precipitation method is combined with HPLC-MS / MS to determine the content of the nine antiepileptic drugs in the plasma. The determination method of the nine antiepileptic drugs in the plasma is fully verified from the aspects of specificity, linearity, sensitivity, accuracy, precision, matrix effect, recovery rate, stability and the like; finally, the method is applied to animal pharmacokinetic research of oral medication, can calculate pharmacokinetic parameters through a non-atrioventricular model, can give the real level of the real concentration of the medicine for treating epilepsy in the body of a patient and can guide doctors to reasonably formulate a drug administration scheme, and therefore, the patient can reasonably take the medicine, side effects can be reduced; and the method has important significance on achieving the optimal curative effect of the patient.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a method for simultaneously measuring the contents of multiple antiepileptic drugs in blood and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Epilepsy, commonly known as "epilepsy madness", is a common clinical disease. Its etiology is complex and diverse. It is a chronic disease with recurrent epileptic seizures caused by highly synchronized discharge of neurons. Systemic dysfunction, often manifested as sudden muscle twitches or loss of consciousness, and is characterized by recurrent attacks. Epilepsy can be seen in all ages,...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/045
Inventor 冯振景叶松弭兆元
Owner JINAN YING SHENG BIOTECH
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