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Composition for efficiently inducing and amplifying human peripheral blood killer immune cells, kit and culture method of immune cells

A technology of immune cells and culture methods, which is applied in the field of compositions for efficiently inducing and expanding killer immune cells in human peripheral blood, which can solve the problems of reduced curative effect of drugs, unsatisfactory effects, and influence on the normal function of the immune system, and achieve the goal of easy use Effect

Pending Publication Date: 2021-06-29
重庆福美干细胞生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are clinical precedents of using PD1 antibody to treat tumor cells, but the effect is not ideal. The main reason is that the human body is a complex system. After entering the human body, PD1 antibody will bind to all immune cells (including killer T cells, immune regulatory T cells) and NK cells, etc.) lead to a decrease in the efficacy of the drug, and at the same time affect the normal function of the entire immune system

Method used

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  • Composition for efficiently inducing and amplifying human peripheral blood killer immune cells, kit and culture method of immune cells
  • Composition for efficiently inducing and amplifying human peripheral blood killer immune cells, kit and culture method of immune cells
  • Composition for efficiently inducing and amplifying human peripheral blood killer immune cells, kit and culture method of immune cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The culturing steps of PDCT cells (T cells+DC cells) provided by the present invention are as follows:

[0055] 1. Collection of peripheral blood mononuclear cells

[0056] Use a blood cell separator to collect the patient's own peripheral blood mononuclear cells 1-2*10 8 (or draw 80ml of autologous peripheral blood).

[0057] 2. Inactivated plasma

[0058] 2.1. Add the obtained peripheral blood into a 50ml centrifuge tube and centrifuge at 1000g for 5min;

[0059] 2.2. Use a pipette to carefully extract 10ml of the upper layer of plasma (do not draw to the boundary layer), add 10ml of normal saline to the centrifuge tube, and obtain peripheral blood and 10ml of plasma after plasma extraction;

[0060] 2.3. Inactivate 10ml of the obtained plasma in a water bath at 56°C for 30 minutes;

[0061] 2.4. Take out the inactivated plasma, put it into a centrifuge tube, centrifuge at 2000g for 5min, take the supernatant, and put it in a 4°C refrigerator for later use.

[00...

Embodiment 2

[0121] The culturing steps of PDCT cells (T cells+DC cells) provided by the present invention are as follows:

[0122] 1. Collection of peripheral blood mononuclear cells

[0123] Use a blood cell separator to collect the patient's own peripheral blood mononuclear cells 1-2*10 8 (or draw 80ml of autologous peripheral blood).

[0124] 2. Inactivated plasma

[0125] 2.1. Add the obtained peripheral blood into a 50ml centrifuge tube and centrifuge at 1000g for 5min;

[0126] 2.2. Use a pipette to carefully extract 10ml of the upper layer of plasma (do not draw to the boundary layer), add 10ml of normal saline to the centrifuge tube, and obtain peripheral blood and 10ml of plasma after plasma extraction;

[0127] 2.3. Inactivate 10ml of the obtained plasma in a water bath at 56°C for 30 minutes;

[0128] 2.4. Take out the inactivated plasma, put it into a centrifuge tube, centrifuge at 2000g for 5min, take the supernatant, and put it in a 4°C refrigerator for later use.

[01...

Embodiment 3

[0188] The culturing steps of PDCT cells (T cells+DC cells) provided by the present invention are as follows:

[0189] 1. Collection of peripheral blood mononuclear cells

[0190] Use a blood cell separator to collect the patient's own peripheral blood mononuclear cells 1-2*10 8 (or draw 80ml of autologous peripheral blood).

[0191] 2. Inactivated plasma

[0192] 2.1. Add the obtained peripheral blood into a 50ml centrifuge tube and centrifuge at 1000g for 5min;

[0193] 2.2. Use a pipette to carefully extract 10ml of the upper layer of plasma (do not draw to the boundary layer), add 10ml of normal saline to the centrifuge tube, and obtain peripheral blood and 10ml of plasma after plasma extraction;

[0194] 2.3. Inactivate 10ml of the obtained plasma in a water bath at 56°C for 30 minutes;

[0195] 2.4. Take out the inactivated plasma, put it into a centrifuge tube, centrifuge at 2000g for 5min, take the supernatant, and put it in a 4°C refrigerator for later use.

[01...

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PUM

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Abstract

The invention discloses a composition for efficiently inducing and amplifying human peripheral blood killer immune cells, a kit and a culture method of the immune cells. The composition is prepared from interleukin-1 beta, interleukin-6, prostaglandin E2, interferon gamma, a CD3 monoclonal antibody, a CD28 monoclonal antibody, interleukin-2, a tumor necrosis factor-alpha, a granulocyte macrophage stimulating factor, interleukin-1 alpha, interleukin-4 and a PD-1 antibody or a CTLA-4 antibody. The composition provided by the invention can effectively improve the multiplication capacity of killer T autoimmune cells and the tumor cell killing capacity of the killer T autoimmune cells.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a composition, a kit and a method for cultivating the immune cells for efficiently inducing and expanding killer immune cells in human peripheral blood. Background technique [0002] The immune system (Immune System) executes the immune response and immune function in the body, and is composed of immune organs, immune cells and immune molecules. The immune system recognizes and eliminates antigenic foreign bodies, coordinates with other systems of the body, and jointly maintains the stability and physiological balance of the body's internal environment. [0003] Thymus dependent lymphocytes, T cells for short, are a kind of immune cells, mainly including killer T cells and memory T cells. Mature T cells are distributed to peripheral immune organs through the blood, and can be recirculated through lymphatic vessels, peripheral blood, and tissue fluid to exert cellular immunit...

Claims

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Application Information

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IPC IPC(8): C12N5/0783C12N5/0784
CPCC12N5/0638C12N5/0636C12N5/0639C12N2501/2301C12N2501/2306C12N2501/02C12N2501/24C12N2501/515C12N2501/51C12N2501/2302C12N2501/25C12N2501/22C12N2501/2304C12N2500/32C12N2501/998
Inventor 李伟强邵文陶
Owner 重庆福美干细胞生物科技发展有限公司
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