Method for breeding male sterile line of Haiyan brassica napobrassica and application

A technology of male sterile line and kohlrabi, applied in the fields of application, botany equipment and method, plant gene improvement, etc., can solve the problems of single species, no sea salt kohlrabi breeding and application, etc.

Active Publication Date: 2021-07-06
ZHEJIANG JIAXING AGRI SCI ACADEMY INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As a local farm variety, sea salt kohlrabi is currently the main method of saving seeds by farmers themselves. The variety is relatively single, and there is a lot of room for improvement in terms of disease resistance, yield, and quality.
The use of heterosis is an effective way to improve crop yield, resistance and quality. It has been widely used in cruciferous vegetables, and the use of male sterile lines to produce hybrids is a common method for breeding new varieties of cruciferous crops. method, there is no report on the selection and application of sea salt kohlrabi male sterile line

Method used

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  • Method for breeding male sterile line of Haiyan brassica napobrassica and application
  • Method for breeding male sterile line of Haiyan brassica napobrassica and application
  • Method for breeding male sterile line of Haiyan brassica napobrassica and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1 sea salt kohlrabi male sterile line breeding method

[0055] 1. Sterility source selection and intermediate vector transfer

[0056] For two consecutive years in 2009 and 2010, the self-owned Brassica napus ogu cytoplasmic male sterile line CMS'92758A' was crossed with local varieties of mustard such as Tongxiang mustard, Huangyan mustard and sea salt kohlrabi, all of which showed the phenomenon of hybridization.

[0057] In 2011, we continued to expand the scope to combine Brassica napus ogu cytoplasmic male sterile line CMS'92758A' with wild-type mustard (tentatively named No. The other types of mustard were crossed, and 52 hybrid seeds were obtained only by crossing with Ye mustard No. 1. Therefore, the ogu cytoplasmic male sterile line of Brassica napus was selected as the sterile source A; the local wild-type mustard C (namely No. Obtained 52 grains (A×C)F 1 30 of the hybrid seeds.

[0058] 2. Purification and rejuvenation

[0059] In 2008, some l...

Embodiment 2

[0071] Embodiment 2 The sea salt kohlrabi male sterile line breeding sterile line seed obtained by the selection of embodiment 1

[0072] In the greenhouse in 2019, the salt kohlrabi male sterile line 17 mustard A (that is, the sterile line kohlrabi A') obtained in Example 1: 17 mustard B (that is, the maintainer line kohlrabi B') was interlaced in a manner of 4:1. to plant. Carry out insect-proof net isolation before the flowering period, bee pollination, remove the kohlrabi B' plant in the early stage of maturity, and keep the kohlrabi A' to harvest some sterile line seeds. According to statistics, the effective rate of siliques in seed production of CMS lines is 72.32%, and the number of siliques per silique is 16.18. The seed setting coefficient of CMS lines is high.

Embodiment 3

[0073] Embodiment 3 The sea salt kohlrabi male sterile line hybridization seed production obtained by the selection and breeding of embodiment 1

[0074] In 2019, the Sichuan farm kohlrabi cultivar 'Badhead mustard' was introduced as the male parent, and the salt kohlrabi male sterile line 17 mustard A (ie male sterile line kohlrabi A') obtained in Example 1 was used as the female parent for hybrid seed production. In the greenhouse, the male parent: the female parent ratio is 1:1, and the interlaced planting is carried out. Before the flowering period, the insect-proof net is isolated, and the dense net is used to prevent other varieties from pollinating and pollinating by bees. The bald mustard plants are removed at the final flowering stage, and the remaining plants are harvested as hybrid seeds. Several hybrids will be harvested in the spring of 2020. According to statistics, the effective pod rate of hybrid seed production is 67.32%, the number of pods per pod is 17.20, ...

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Abstract

The invention discloses a method for breeding a male sterile line of Haiyan brassica napobrassica. The method comprises four links of selection of a male sterile line sterile source, utilization of the sterile source for transforming an intermediate vector, selection and purification of a Haiyan brassica napobrassica farm variety, and backcross breeding of the Haiyan brassica napobrassica male sterile line. According to the method, the sterile source comes from a cabbage type rape ogu cytoplasmic male sterile line, a backcross transformation parent is a Haiyan brassica napobrassica purified strain, and a wild type mustard variety is selected as the intermediate vector. According to the method provided by the technical scheme, the problems of flowering asynchronism and hybridization incompatibility in direct hybridization of the Haiyan brassica napobrassica sterile line and the cabbage type rape sterile line can be effectively solved, and the bred sterile line is thorough in sterility, good in floral organ development and normal in nectary development. According to application of the male sterile line of the Haiyan brassica napobrassica obtained through breeding by the method, the seed reproduction rate is high, the heterosis is obvious, and the method has important significance for subsequent breeding and utilization of high-quality hybrids of the Haiyan brassica napobrassica.

Description

technical field [0001] The invention relates to the technical field of germplasm innovation, in particular to a method and application of sea salt kohlrabi male sterile line breeding. Background technique [0002] Sea salt kohlrabi belongs to Brassica (B.juncea L.) kohlrabi variant of Cruciferae (Cruciferae), belongs to root mustard in agronomic classification, is a special product of Haiyan County, Zhejiang Province, and a product of China's national geographical indication. Sea salt kohlrabi, also known as "flat big species", is named for its flat leaves at noon. Under the specific geographical conditions of Haiyan and the specific local agricultural production habits, the fresh vegetables produced in the protected area of ​​Haiyan kohlrabi have a smooth surface, large roots, high yield, less hard tendons inside, and the processed kohlrabi tastes delicious and crispy . [0003] As a local farm variety, sea salt kohlrabi is currently the main method of saving seeds by far...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 姚祥坦张敏王瑞森沈盟袁晔权新华马燕欣胡彬慧
Owner ZHEJIANG JIAXING AGRI SCI ACADEMY INST
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