Traditional Chinese medicine composition for reducing somatic cell count in milk and preparation method thereof

A somatic cell and composition technology, which is applied in the field of traditional Chinese medicine compositions for reducing the number of somatic cells in milk and the field of preparation thereof, can solve the problems of reducing the number of somatic cells in milk, reducing the milk body, and failing to repair damage to breast tissue, etc. The effect of reducing the number of milk somatic cells, reducing the number of somatic cells, and repairing breast tissue damage

Pending Publication Date: 2021-07-16

章国忠

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AI-Extracted Technical Summary

Problems solved by technology

[0009] Antibiotic treatment and traditional Chinese medicine clearing heat and removing blood stasis have no effect on repairing the damage of breast tissue, so they are not effective in reducing the number of somatic cells in milk

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Abstract

The invention provides a traditional Chinese medicine composition for reducing the number of somatic cells in milk and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following ingredients in parts by mass: 0.05 to 20 parts of radix angelicae sinensis, 0.05 to 20 parts of radix paeoniae rubra, 0.05 to 20 parts of radix astragali seu hedysari, 0.05 to 20 parts of radix codonopsis, 0.05 to 20 parts of radix rehmanniae, 0.05 to 20 parts of radix scrophulariae, 0.05 to 20 parts of cortex cinnamomi, 0.05 to 20 parts of radix aconiti carmichaeli preparata, 0.1 to 40 parts of radix et rhizoma rhei, 0.1 to 40 parts of tribulus terrestrisL, 0.05 to 20 parts of semen coicis, 0.05 to 20 parts of radix crotonis crassifolii, 0.05 to 20 parts of rhizoma coptidis, 0.05 to 20 parts of caulis akebiae, 0.05 to 20 parts of fructus aurantii immaturus and 0.05 to 20 parts of herba patriniae. The traditional Chinese medicine composition can effectively treat clinical mastitis and recessive mastitis of dairy cows, repair breast tissue damage and achieve the effect of reducing the number of somatic cells in milk.

Application Domain

Pharmaceutical delivery mechanismSexual disorder +1

Technology Topic

Somatic cellBiotechnology +15

Image

Examples

Experimental program(3)

Example Embodiment

[0030] Example 1

[0031] The preparation step of the preparation of the Chinese medicine composition of the present invention in the filling agent type of the present invention is as follows:

[0032] Step 1: After the raw material weighing, the raw material is pulverized, placed in the microwave extraction equipment, heated to the surface temperature of 60 ° C, repeating three microwave radiation, then add purified water with the material quality equal amount, reflux filtration, The filtrate was allowed to stand for A liquid, spare;

[0033] Step 2: Take the steps after the extraction treatment, the normal pressure low temperature dry displacement is performed, and the low temperature temperature is 100 to 120 ° C, and the retardation is retired under 135 ° C oil bath, and the total distillation The distillate was allowed to stand for 48 h to give a B liquid, spare;

[0034] Step 3: Mix the A liquid and the B liquid uniform, then filtered after 1 h at a temperature of 95 to 100 ° C, adjust the filtrate pH to 6.0, stand for 24 h, filter can be filtered, that is, the perfusion dosage form is reduced milk Chinese medicine compositions of somatic cells.

Example Embodiment

[0035] Example 2

[0036] The preparation steps of the Chinese medicine compositions of the oral liquid type of the present invention reduce the number of body cells in milk are as follows:

[0037] Step 1: In accordance with the raw materials weighing, add weight than 10 times the distilled water, soak water for 2 h, decoction twice, the first 2 hours, the second 1h, combined with decoction, filtrate;

[0038] Step 2: The filtrate obtained by the step is concentrated under reduced pressure to a relative density of 1.08, refrigerated 24h, and then filtered, and a 2% acetic acid solution of 1% chitosan is added in a ratio of 10: 1. Clarification, filtration, filtrate, sodium benzoate, add water to 1000 ml, mix uniform, fill, sterilize, and add oral liquid dosage forms to reduce milk in milk. Chinese medicine composition.

Example Embodiment

[0039] Example 3

[0040] The preparation steps of the Chinese medicine composition of the particulate dosage form of the present invention have the preparation of the Chinese medicine composition of the milk in milk are as follows:

[0041] Step 1: In accordance with the raw materials weighing, add weight than 10 times the distilled water, soak water for 2 h, decoction twice, the first 2 hours, the second 1h, combined with decoction, filtrate;

[0042] Step 2: The filtrate obtained by the step is concentrated under reduced pressure at 60 ° C to a relative density of 1.28-1.30 to obtain a cleaner, and then the proportion of cleans, sugar, and dextrin is made into particles. , 350 g of a Chinese medicine composition having a particulate agent type reduced milk in milk is obtained.

[0043] The properties of the Chinese medicine composition perfusion prepared by experiments were detected by experiment.

[0044] Animal grouping and administration

[0045] 200 pieces of cows in milk in milk in milk were selected, randomly divided into two groups, wherein I group I group was a penicillin therapeutic test group, and II group was a pharmaceutically acceptable liquid treatment test group.

[0046] Group I: Muscle injection of penicillin, 2-30,000 units / kg, 2 times a day, 3-7 days of treatment;

[0047] Group II: 10ml per breast in each cow, 2 times a day, 3-7 days.

[0048] 2. Diagnostic criteria of the number of milk in milk

[0049] The choice of cows in milk in milk has the following symptoms, indicators or some symptoms indicators can be diagnosed as exceeding the standard.

[0050] a. The size of the breast is increased, the heat, the texture is hard; B. Breast skin is red, the palpation has pain; c. Each decauli area is different, the lymph nodes of the breasts are large; D. Latex color, viscous, floc Abnormalities, curd blocks, blood clots, etc. e. California breastitis test CMT strong positive; F. US Bently Soma Count counter automatically detects, the number of milk in the milk is 5 million / ml.

[0051] 3. Evaluation criteria for lowering the number of milk cells

[0052] Cure: The clinical symptoms disappear, intuitive milk has no abnormalities, basic milk production, CAT method is lower than positive "++", the US Bently Soma Count counter automatically detects, milk neutral cell number is 500,000 / Ml or less, for cure.

[0053] How to improve or disappear, there is a small amount of flocculation in the milk, but the milk has no abnormality, the production milk is not normal, and the test results of CMT method in California are above positive "++", US Bently Soma count The counter automatically detects that the number of milk in the milk is 1500,000 / ml, which is improved.

[0054] Invalid: The clinical symptoms and indicators in the judgment standard did not alleviate or intensified.

[0055] Efficiency: effective rate = (healed + productive) / total case number * 100%.

[0056] 4. Experimental results

[0057] The results of the clinical treatment test of each test group on the number of cow's number of milk cells were as follows.

[0058] Table 1

[0059]

[0060] 5. Experimental Conclusion

[0061] The results showed that the present invention is better than the curative effect of penicillin, and the cure rate and efficiency of the number of milk cells are reduced by treating breast inflammation. It is more efficient to 95%. The traditional Chinese medicine composition of the present invention provides an efficient, low-cost, simple, safe, and residual drug, with a broad market prospect to reduce the number of milk cells.

[0062] Thus, the Chinese medicine composition of the present invention can effectively treat cow clinical breastitis and recessive breast inflammation, and repair breast tissue damage to reduce the number of somatic cells in milk.

PUM

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