Multi-target detection method for blood sample

Abstract

The invention relates to a multi-target detection method for a blood sample, which comprises the following steps: 1) preparing a reagent for reaction, wherein the reagent comprises a coding magnetic microsphere series, and the surface of the coding magnetic microsphere is connected with a specific capture antibody; wherein the encoding magnetic microsphere series comprises magnetic microspheres with different particle sizes, the particle sizes of the magnetic microspheres can be distinguished by a signal of a flow analyzer, and the encoding magnetic microsphere series is used for identifying the types of the microspheres; 2) carrying out reaction and treatment on a blood sample to be detected and a reagent, and removing interference components to form a fluorescent labeled reaction compound based on microspheres; and 3) sending the reaction compound into a flow optical detection system for analysis, obtaining a characteristic signal, identifying the type of the microspheres, and simultaneously detecting multiple targets in the blood sample. Compared with the prior art, the method has the advantages that the coding microspheres have stable characteristics, a whole blood sample is used, multiple detection is realized, and the like.

Description

technical field [0001] The invention relates to the field of biomolecular analysis and detection, in particular to a multi-target detection method for blood samples. Background technique [0002] Now, a large number of biomolecular analyzes in clinical diagnosis, such as protein, nucleic acid, etc., are usually detected on biochemical analyzers, chemiluminescence immunoassay analyzers and other equipment, using serum or plasma samples, but the preparation of serum or plasma requires coagulation and Centrifugal operation consumes a lot of time and manpower. [0003] For acute diseases, the rapid detection of characteristic proteins uses whole blood samples, such as specific protein analyzers, but specific protein analyzers usually use immune turbidimetry, such as immune transmission or immune scattering turbidimetry. Due to the limitation of the detection principle, turbidimetric The detection sensitivity and precision of the method are not high. [0004] The objects detect...

AI Technical Summary

Problems solved by technology

[0016] 1. The cost of fluorescent microspheres is high, the production process is fine and complex, and the fluorescence stability is not good;

[0017] 2. The cost of the instrument is high, the optical adjustment is difficult, and the electronic and software processing is complicated:

[0021] 3. There are too many types of microspheres in multiple detection reagents. If there are dozens of types, the risk of non-specific binding and cross-interference of detection items is high. The specificity requirements for detection antibodies are very high, and development and verification are very difficult. Big

[0027] Difficulties and challenges of using whole blood samples. Compared with serum samples, whole blood samples contain a large number of blood cells and various impurities, causing non-specific interference, and the size of blood cells is close to the size of microspheres. In flow optical detection Causes a lot of interference to the screening of microspheres

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