Method for establishing fingerprint spectrum of medicinal preparation of decumbent bugle herb

A technology of pharmaceutical preparations and fingerprints, which is applied in pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., can solve problems such as singleness, and achieve the effects of avoiding interference, good repeatability, and increasing specificity.

Active Publication Date: 2021-07-23
CHINA RESOURCES SANJIU MEDICAL & PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]Therefore, the technical problem to be solved in the present invention is to overcome the defect of only single content determination (harpaside, acetylharpaside content determination) in Herba Japonica in the prior art , For this reason, the first aspect: the present invention proposes a kind of method that establishes the fingerprint of the medicinal preparation of Herba Sinensis, the method comprises the steps:

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  • Method for establishing fingerprint spectrum of medicinal preparation of decumbent bugle herb
  • Method for establishing fingerprint spectrum of medicinal preparation of decumbent bugle herb
  • Method for establishing fingerprint spectrum of medicinal preparation of decumbent bugle herb

Examples

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Effect test

Embodiment 1

[0073] In the following examples and experimental examples, the preparation method of the Herbs sinensis formula granules is as follows: take the Herbs sinensis, heat and reflux extraction twice, add 12 times the amount of water by weight for the first time to extract for 30 minutes, and add 10 times the amount of water by weight for the second time Extract for 25 minutes, filter, combine the filtrates, concentrate the filtrate to a relative density of 1.06-1.12 at 60°C, add conventional excipients, and make granules according to conventional processes.

Embodiment 2

[0075] The present embodiment establishes the method for the finger-print of the Herb Grass Formula Granules, comprising the following steps:

[0076] (1) Take 0.4g of the medicinal preparation of Herba sinensis to be tested, accurately weighed, accurately add 25mL of 50% (v / v) methanol aqueous solution, weigh the weight, extract by ultrasonic for 30 minutes, let it cool, weigh again, take 50 % (v / v) methanol aqueous solution to supplement the lost weight, shake up, filter, and get the subsequent filtrate as the test solution;

[0077] (2) Accurately weigh the reference substance of harpaside, add 50% (v / v) methanol aqueous solution to make a solution containing 0.0002g per 1mL, shake well, and use it as the reference substance A solution;

[0078] Accurately weigh the acetylharpaside reference substance, add 50% (v / v) methanol aqueous solution to make a solution containing 0.0002g per 1mL, shake well, and use it as the reference substance B solution;

[0079] (3) Chromatogra...

Embodiment 3

[0083] The present embodiment establishes the method for the finger-print of the Herb Grass Formula Granules, comprising the following steps:

[0084] (1) Take 0.3 g of the medicinal preparation of Herba chinensis to be tested, accurately weighed, accurately add 20 mL of 60% (v / v) methanol aqueous solution, weigh the weight, extract under reflux for 40 minutes, let it cool, weigh again, and take 60% (v / v) methanol aqueous solution to make up the lost weight, shake well, filter, and get the subsequent filtrate as the test solution;

[0085] (2) Accurately weigh the reference substance of harpaside, add 60% (v / v) aqueous methanol solution, shake well, and use it as the reference substance A solution; each 1mL of the reference substance A solution contains 0.0002g of harpaside;

[0086] Accurately weigh the acetylharpaside reference substance, add 60% (v / v) methanol aqueous solution, shake well, and use it as the reference substance B solution; each 1mL reference substance B solu...

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Abstract

The invention relates to a method for establishing a fingerprint spectrum of a medicinal preparation of decumbent bugle herb. According to the method, decumbent bugle herb formula granules are taken as a detection object, a fingerprint spectrum aiming at the medicinal preparation is established, a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system is adopted to establish a control fingerprint spectrum, eight common characteristic peaks are calibrated, and harpagide at the first peak and acetyl harpagide at the third peak of the common characteristic peaks are confirmed; acetylharpagide at the third peak is selected as an internal reference peak, and the relative retention time of common characteristic peaks of the ajuga ciliata formula granules is determined; and the similarity between fingerprint of the sample to be detected and the contrast fingerprint is evaluated. According to the method disclosed by the invention, the quality of the medicine can be comprehensively and quickly detected, and comprehensive quality detection and overall quality control of the medicine are facilitated, so that the use safety and stability of the medicine are favorably improved. Meanwhile, the method has the advantages of high stability, high precision, high repeatability and the like.

Description

technical field [0001] The invention belongs to the field of drug analysis, and in particular relates to a method for establishing the fingerprint of a medicinal preparation of Herba chinensis. Background technique [0002] Jingucao formula granules are obtained by extracting, concentrating and granulating the Chinese herbal medicine Jingucao. [0003] Muscaria sinensis is the dried whole herb of Ajuga decumbens Thunb., a plant of the Lamiaceae family. It mainly contains various components such as crodanes, diterpenoids, iridoids, flavonoids, glycosides and sterones, and has heat-clearing and detoxifying properties. , the effect of cooling blood and reducing swelling, can be used for sore throat, hemoptysis due to lung heat, swelling and pain from falls. At present, there is confusion about the close relatives of Muscaria sinensis, including Jinchuangxiaocao, Zibeijinpan and Prunella vulgaris. At present, there are few studies on the quality standards of Herba japonica. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/60G01N30/74G01N30/86A61K36/53
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/60G01N30/74G01N30/8679G01N30/8686A61K36/53G01N2030/324A61K2236/331A61K2236/51A61K2236/39
Inventor 黄凯伟郑晓英张辉谭沛赵伟志仝一丹
Owner CHINA RESOURCES SANJIU MEDICAL & PHARMA
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