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A colloidal gold test strip for detecting human thyroglobulin

A technology of thyroglobulin and test strips, applied in the field of biomedical testing, can solve the problems of large impact, long time, complicated operation, etc., and achieve the effect of shortening the testing time, reducing the workload, and being convenient and fast to use

Active Publication Date: 2021-12-07
施康培医药科技(武汉)有限公司
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AI Technical Summary

Problems solved by technology

This detection method is complicated to operate and takes a long time, which is not conducive to clinical operation.
Third, there may be auto-Tg antibodies in peripheral blood, and the presence of auto-Tg antibodies has a greater impact on the quantitative detection of Tg

Method used

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  • A colloidal gold test strip for detecting human thyroglobulin
  • A colloidal gold test strip for detecting human thyroglobulin
  • A colloidal gold test strip for detecting human thyroglobulin

Examples

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Embodiment Construction

[0026] The principles and features of the present invention are described below, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0027] 1. Antibody screening and preparation

[0028] Full-length thyroglobulin (Tg) was used to immunize alpacas to prepare anti-human Tg antibodies. Mix and emulsify 250 μg antigen with 250 μL Freund’s complete adjuvant, and use the mixture to immunize alpacas to complete the primary immunization. After 2 weeks and 4 weeks after the primary immunization, the alpacas were given a booster immunization, and the mixture of 1250 μg antigen and 250 μL Freund's incomplete adjuvant was used for booster immunization. One week after the second and third immunizations, blood was collected to determine the titer of antiserum.

[0029] The results showed that the antiserum titers elicited by the second and third immunizations of the antigen were 2.25×10 6 and 9.43×10 ...

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Abstract

The invention relates to a test paper strip for detecting human thyroglobulin, which comprises a base plate, and a sample pad, a colloidal gold binding pad, a nitrocellulose membrane and a water-absorbing pad arranged on the base plate and sequentially connected, and the nitrocellulose membrane is provided with a detection line; the colloidal gold binding pad contains colloidal gold particles labeled with binding antibodies; the detection line is anchored with a capture antibody, and both the binding antibody and the capture antibody can specifically recognize and bind thyroglobulin without interfering with each other. The colloidal gold test strip of the present invention is convenient and quick to use, can complete the chromatographic reaction within 10 minutes, and is used for quantitative detection to help doctors judge whether the Tg content in peripheral blood exceeds the normal range in a short time. Semi-quantitative detection test strips can be used by patients themselves, which is convenient for patients to monitor themselves daily. The dual-function test strip can eliminate test samples whose Tg content is within the normal range, greatly reducing the workload of clinical testing.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a colloidal gold test strip for detecting human thyroglobulin. Background technique [0002] Thyroglobulin (Tg) is a glycoprotein secreted by thyroid follicular epithelial cells, with a molecular weight of about 660kD. play an important role in the biosynthesis of . In a healthy person, only a very small amount of Tg enters the blood circulation, and the recommended normal value of Tg is 3-40ng / ml. Some diseases such as differentiated thyroid cancer (DTC) can cause elevated Tg levels in peripheral blood. Therefore, Tg is a key serological indicator of DTC, which can assist in the initial diagnosis and diagnosis of DTC. Regular follow-up of Tg levels after treatment can assist in monitoring the prognosis and recurrence risk of DTC patients. [0003] At present, there are some technical problems in the determination of Tg content. First of all, there a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/558G01N33/58G01N33/68G01N33/577
CPCG01N33/558G01N33/57407G01N33/57488G01N33/577G01N33/587G01N33/68G01N2333/4713
Inventor 朱富强王珏
Owner 施康培医药科技(武汉)有限公司
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