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Agaricus bisporus mother strain culture medium and application thereof

A technology of Agaricus bisporus and culture medium, applied in the field of microbial culture, can solve the problems of easy degradation of strains, long culture time, poor strain growth, etc., and achieves dense and neat mycelium, fast mycelium growth, and increased resource utilization. The effect of using

Active Publication Date: 2021-08-06
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The parent culture medium of Agaricus bisporus usually adopts PDA medium, but when PDA medium is used for the propagation of Agaricus bisporus strains, not only the cultivation time is long, the growth of the strains is also poor, and the strains are easy to degenerate

Method used

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  • Agaricus bisporus mother strain culture medium and application thereof
  • Agaricus bisporus mother strain culture medium and application thereof
  • Agaricus bisporus mother strain culture medium and application thereof

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Embodiment 1

[0029] In the previous research of the applicant, it was found that in the prior art, the medium formula was obtained through the compounding of orthogonal experiments, and the operation method was very complicated, and the waste residue of edible fungus cultivation contained various components beneficial to the growth of edible fungi, and During the cultivation process of edible fungi, the culture material is designed according to the characteristics of the edible fungus itself, and the use of edible fungus residue can shorten the research time for the combination of raw materials; : Not all edible fungus waste residues are suitable for cultivating edible fungus parent species, because the edible fungus waste residue itself has undergone utilization and absorption by edible fungi, and its nutrient composition and structure have changed greatly, so it is difficult to use it to cultivate parent species. There is a problem of adaptability to the mother species. For this reason, w...

Embodiment 2

[0055] This embodiment studies the growth situation of Agaricus bisporus parent species under different contents of Agaricus bisporus slag, and the specific culture medium is as follows:

[0056] Wheat grain PDA medium (CK6): 200g wheat grain, 200g potato, 20g glucose, 20g agar powder, 1000ml water to volume, pH8.0.

[0057] Corn flour PDA medium (CK7): corn flour 20g, potato 200g, glucose 20g, agar powder 20g, water to volume 1000ml, pH8.0.

[0058] Mushroom residue PDA medium (treatment 1): Agaricus bisporus residue 50g, potato 200g, glucose 20g, agar powder 20g, water to volume 1000ml, pH8.0.

[0059] Mushroom residue PDA medium (treatment 2): Agaricus bisporus residue 100g, potato 200g, glucose 20g, agar powder 20g, water constant volume 1000ml, pH8.0.

[0060] Mushroom residue PDA medium (treatment 3): Agaricus bisporus residue 200g, potato 200g, glucose 20g, agar powder 20g, water constant volume 1000ml, pH8.0.

[0061] The preparation method of above-mentioned wheat g...

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Abstract

The invention relates to the technical field of microbial culture, in particular to an agaricus bisporus mother strain culture medium and application thereof. The agaricus bisporus mother strain culture medium is improved by adding mushroom residue juice into a PDA culture medium, and a very good mother strain culture effect is achieved; and according to the mother strain culture medium, mycelia grow fast and are very dense and neat; compared with other culture media, only one more component, namely the mushroom residue extracting solution, is added into the PDA culture medium, the preparation steps are simple and convenient, and a very good strain culture effect can be achieved. The mushroom residues used in the invention are residual mushroom residues after cultivation of agaricus bisporus, and the raw material source is sufficient; and the agaricus bisporus mother strain culture medium is prepared from the mushroom residues, so that a new way for resource utilization of the mushroom residues is also increased.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganism cultivation, in particular to a culture medium of Agaricus bisporus mother species and its application. 【Background technique】 [0002] Agaricus bisporus, commonly known as mushrooms, white mushrooms, and sea mushrooms, is an edible fungus species with the largest artificial cultivation scale and the most widely distributed production areas in the world. The mother species of Agaricus bisporus is the primary provenance of Agaricus bisporus, and the quality of the mother species is directly related to the yield and quality of Agaricus bisporus cultivation. The mother seed medium is a medium used for the preservation and propagation of Agaricus bisporus strains. The parent culture medium of Agaricus bisporus usually adopts PDA medium, but when using PDA medium to propagate the strains of Agaricus bisporus, not only the cultivation time is long, but also the strain growth is poor, and the st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20
CPCA01G18/20
Inventor 吴圣进陈雪凤张雯龙刘增亮王灿琴韦仕岩
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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