Method for producing yolk antibody for prevention or treatment of obesity through cholesterol absorption inhibition

A technology of egg yolk antibody and cholesterol, which is applied in the direction of antibodies, antibody medical components, chemical instruments and methods, etc., can solve the problems of insufficient development of obesity therapeutic agents and obstacles to cholesterol absorption, and achieve inhibition of absorption, prevention or treatment of obesity, specificity and High binding effect

Pending Publication Date: 2021-08-06
(株)AD生物技术
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  • Summary
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  • Description
  • Claims
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Problems solved by technology

As the risk of various syndromes caused by such obesity is reported, attention to obesity treatment is increasing worldwide, however, in fact, the development of obesity therapeutic agents showing significant effects is still insufficient
[0008] Recently, as an obesity therapeutic agent for treating obesity by inhibiting the absorption of cholesterol, an ezetimibe compound known as Zetia has been used, however, various obesity therapeutic agents containing it Informed that various side effects such as rash, allergy, dry mouth, paresthesia, constipation, insomnia, nausea, dizziness, insomnia, etc.

Method used

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  • Method for producing yolk antibody for prevention or treatment of obesity through cholesterol absorption inhibition
  • Method for producing yolk antibody for prevention or treatment of obesity through cholesterol absorption inhibition
  • Method for producing yolk antibody for prevention or treatment of obesity through cholesterol absorption inhibition

Examples

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Effect test

preparation example 2

[0047] Preparation Example 2: Preparation of Mouse Antibody

[0048] Toxicity was suppressed by inactivating the antigen produced in Preparation Example 1 above, and it was stored in a refrigerator before use. A mouse antibody preparation vaccine was prepared using a complete adjuvant. Vaccines were prepared for each antigen and antibodies were prepared according to conventional mouse antibody preparation methods. Mouse antibodies were purified using a protein-A column from the recovered serum (Serum) after production.

preparation example 3

[0049] Preparation Example 3: Preparation of Antigen-Heterologous Antibody Complex

[0050] In order to bind the antigen produced in Preparation Example 1 above with the mouse antibody produced in Preparation Example 2, the following experiments were carried out. 100 μg of mouse antibodies produced against each antigen were mixed with the inactivated mixed vaccine, and antigen-antibody binding was induced at 4° C. overnight. The combined sample was centrifuged and recovered, and used as a sample for inoculation of laying hens.

preparation example 4

[0051] Preparation example 4: Preparation of vaccine for laying hens inoculated

[0052] In order to prepare a mixed vaccine, mix each antigen and monoclonal antibody complex (mAbcomplex) prepared in Preparation Example 1 with the adjuvant (adjuvant) at a ratio of about 3:7, and then use a homogenizer (Homogenizer) to prepare a vaccine After the live vaccine, the vaccine mix for the production of specific yolk antibodies is prepared by sterility testing and inactivation confirmation tests.

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Abstract

The present invention relates to a method for producing a yolk antibody for prevention or treatment of obesity through cholesterol absorption inhibition, in which an antigen prepared using a Niemann-Pick C1-Like 1 (NPC1L1) recombinant protein is inoculated into an animal heterogeneous to chickens to afford an heterogenetic antibody and a complex having the antigen and the heterogenetic antibody conjugated to each other is inoculated into a layer chicken to produce a yolk antibody. According to the present invention, the method for producing a yolk antibody for prevention or treatment of obesity through cholesterol absorption inhibition is designed to inject the antigen-heterogenetic antibody complex into layer chickens and thus can generate and produce a yolk antibody on a large scale. The yolk antibody thus produced has high specificity and binding affinity for the Niemann-Pick C1-Like 1 (NPC1L1) recombinant protein and blocks the function of the NPC1L1 protein to inhibit cholesterol absorption into the gut. Consequently, the yolk antibody can prevent or treat obesity.

Description

technical field [0001] The present invention relates to a method for preparing an egg yolk antibody for preventing or treating obesity by inhibiting cholesterol absorption, and more particularly, to a method for preparing an egg yolk antibody for preventing or treating obesity by inhibiting cholesterol absorption, that is, by After the antigen prepared by using the recombinant protein of Niemann-Pick C1-Like1 (Niemann-Pick C1-Like1, NPC1L1) was inoculated in an animal belonging to a different species of chicken to prepare heterologous antibodies, the above-mentioned antigen and heterologous The antibody-bound complex is inoculated into laying hens to prepare egg yolk antibodies. Background technique [0002] Antibody is a globulin protein formed by B lymphocytes or B cells in lymphoid tissue, and it is one of the important substances that interfere with specific immune recognition. Antibodies have two different functions. The first function is to neutralize the effect of an...

Claims

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Application Information

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IPC IPC(8): C07K16/18C07K16/02A61P3/04
CPCC07K16/18C07K16/02A61P3/04C07K2317/11C07K2317/76A61K39/00C07K16/28A61K2039/505
Inventor 郑洪杰白斗渊元美庆
Owner (株)AD生物技术
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