Method for producing yolk antibody for prevention or treatment of obesity through cholesterol absorption inhibition
A technology of egg yolk antibody and cholesterol, which is applied in the direction of antibodies, antibody medical components, chemical instruments and methods, etc., can solve the problems of insufficient development of obesity therapeutic agents and obstacles to cholesterol absorption, and achieve inhibition of absorption, prevention or treatment of obesity, specificity and High binding effect
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preparation example 2
[0047] Preparation Example 2: Preparation of Mouse Antibody
[0048] Toxicity was suppressed by inactivating the antigen produced in Preparation Example 1 above, and it was stored in a refrigerator before use. A mouse antibody preparation vaccine was prepared using a complete adjuvant. Vaccines were prepared for each antigen and antibodies were prepared according to conventional mouse antibody preparation methods. Mouse antibodies were purified using a protein-A column from the recovered serum (Serum) after production.
preparation example 3
[0049] Preparation Example 3: Preparation of Antigen-Heterologous Antibody Complex
[0050] In order to bind the antigen produced in Preparation Example 1 above with the mouse antibody produced in Preparation Example 2, the following experiments were carried out. 100 μg of mouse antibodies produced against each antigen were mixed with the inactivated mixed vaccine, and antigen-antibody binding was induced at 4° C. overnight. The combined sample was centrifuged and recovered, and used as a sample for inoculation of laying hens.
preparation example 4
[0051] Preparation example 4: Preparation of vaccine for laying hens inoculated
[0052] In order to prepare a mixed vaccine, mix each antigen and monoclonal antibody complex (mAbcomplex) prepared in Preparation Example 1 with the adjuvant (adjuvant) at a ratio of about 3:7, and then use a homogenizer (Homogenizer) to prepare a vaccine After the live vaccine, the vaccine mix for the production of specific yolk antibodies is prepared by sterility testing and inactivation confirmation tests.
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