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Method for extracting total phenols of alfalfa by optimizing ultrasonic-assisted method through response surface method

A response surface method and ultrasonic technology, applied in the direction of organic chemistry, can solve the problems that are not suitable for rapid detection and large-scale extraction, large-scale extraction cannot be carried out, and there are many precautions, so as to shorten the extraction time, reduce the cost and increase the extraction. rate effect

Pending Publication Date: 2021-09-07
GANSU AGRI UNIV
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Problems solved by technology

Although the ultrasonic-assisted acetone extraction method improves the total phenol extraction, acetone is a dangerous chemical, flammable, easy to produce poison, and extremely harmful to human body
In addition, due to the particularity of the instrument, the microwave-assisted extraction method requires more precautions and cannot be used for large-scale extraction; although high-performance liquid chromatography has high accuracy and sensitivity, it is expensive and not suitable for Rapid detection and large-scale extraction

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  • Method for extracting total phenols of alfalfa by optimizing ultrasonic-assisted method through response surface method
  • Method for extracting total phenols of alfalfa by optimizing ultrasonic-assisted method through response surface method
  • Method for extracting total phenols of alfalfa by optimizing ultrasonic-assisted method through response surface method

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Embodiment Construction

[0021] Below in conjunction with accompanying drawing and further illustrate technical scheme of the present invention by specific embodiment:

[0022] The present invention is illustrated below in conjunction with specific embodiments.

[0023] Response surface methodology optimizes the method for ultrasonic-assisted extraction of total phenols from alfalfa, comprising the following steps:

[0024] 1) Alfalfa variety: Gannong No. 9 alfalfa (M. sativa 'Gan-nong No.9').

[0025] 2) Sample acquisition: alfalfa was planted in an 8m×6m×2m room covered with 60 mesh insect-proof nets at the Forage Experimental Base of Gansu Agricultural University. Use 0.1% HgCl to select uniform and full Gannong No. 9 seeds 2 After disinfection, they were sown in holes in the ground, with a plant spacing of 10 cm and a row spacing of 10 cm. Each treatment had 4 rows, and each row had 9 plants, repeated 3 times. Thin out the seedlings after emergence, and keep 3 seedlings with consistent growth a...

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Abstract

The invention relates to the field of plant processing, and in particular, relates to a method for extracting total phenols of alfalfa by optimizing an ultrasonic-assisted method through a response surface method. According to the method, the total phenols are extracted by inoculating thrips to alfalfa to stimulate and analyzing and optimizing a Box-Behnken response surface through an ultrasonic method, and the method is specifically optimally characterized by comprising the steps: adding air-dried and crushed alfalfa into ethanol, and performing ultrasonic extraction, wherein the solid-liquid ratio of the alfalfa to the ethanol is 1:20, the volume fraction of the ethanol is 60%, the extraction time of the alfalfa in the ethanol is 45 minutes, and the extraction temperature is 60 DEG C. The method can effectively shorten the extraction time, increase the extraction rate and reduce the cost.

Description

technical field [0001] The invention relates to the field of plant processing, in particular to a method for optimizing the ultrasonic-assisted method for extracting total phenols from alfalfa by response surface method. Background technique [0002] With the in-depth research on various natural bioactive components of alfalfa (Medicago sativa), such as phenols, flavonoids, saponins, polysaccharides, dietary fiber and vitamin E, coupled with the high yield of alfalfa grassland, the development and utilization of plant functional substances more and more people's attention. The phenolic compounds of alfalfa are a wide variety of compounds with important physiological functions. It not only has strong natural antioxidant properties and a variety of medicinal values, it can be used in natural antioxidants, natural pigments, functional foods, etc.; Under food stimulation conditions, phenolic compounds play an important regulatory role in plant defensive secondary metabolism, si...

Claims

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Application Information

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IPC IPC(8): C07G99/00
CPCC07G99/00
Inventor 吴芳师尚礼康文娟李兴龙黄宗昌张辉辉
Owner GANSU AGRI UNIV
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