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Extraction method of phenolic substances of Chinese fevervine herb and root as well as application of extraction method

An extraction method, the technology of chrysanthemum chrysogenol, which is applied in the field of chrysanthemum extraction, can solve the problems of flammability, low substance content, and low extraction efficiency, and achieve the effect of accurate detection and high antioxidant activity

Pending Publication Date: 2021-11-05
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, microwave-assisted extraction or traditional organic solvents are mainly used to extract the polyphenols in Chickweed. However, this method has many inherent disadvantages, such as: organic solvents are highly volatile, highly toxic, difficult to be biodegraded, Disadvantages such as flammability; the extraction efficiency of leaching is low, and the extracted substances have low content and few types

Method used

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  • Extraction method of phenolic substances of Chinese fevervine herb and root as well as application of extraction method
  • Extraction method of phenolic substances of Chinese fevervine herb and root as well as application of extraction method
  • Extraction method of phenolic substances of Chinese fevervine herb and root as well as application of extraction method

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Experimental program
Comparison scheme
Effect test

no. 1 example

[0026] A method for extracting gallinaceous phenols, comprising the steps of:

[0027] (1) Preparation of deep eutectic solution: Mix different hydrogen bond acceptors and hydrogen bond donors in proportion, heat them in a water bath at 80°C and keep stirring until a uniform transparent liquid with a certain viscosity is formed, and cool to room temperature ( About 25 ℃), add water and mix well, obtain the depth eutectic solution (DESs) of different components that water content is 40% (mass concentration), specifically as shown in table 1.

[0028] (2) Mix 0.5g of Caulis chinensis powder with 5mL of DESs prepared in step (1), extract the resulting mixture at 40°C with 320W ultrasonic power for 30min, and then extract the obtained crude extract in a centrifuge at 13000rpm Centrifuge at a high speed for 10 minutes, and take the supernatant to obtain the extract of P. chinensis.

[0029] (3) Determination of the total flavonoids content in the extract of Caulis chinensis by alu...

no. 2 example

[0035] Using ChCl-EG in the first embodiment as the deep eutectic solvent (that is, choline chloride and ethylene glycol with a molar ratio of 1:2), it is prepared into a deep eutectic solvent with a water content of 40% (mass concentration). solution, and then adopt the method of step (2) to obtain the extract of Caulis chinensis.

[0036] Further, in step (2), the adopting 2 that is generated by Minitab 17 software K The design matrix constructed by the factorial experimental design comprehensively investigated 5 factors, including the water content of DES (A), the mass-to-volume ratio (B) of the deep eutectic solution and the cassava powder, the ultrasonic power (C), and the ultrasonic time ( D), the effect of ultrasonic temperature (E) and its interaction on the total flavonoid content of the extract of Caulis chinensis. Taking the total flavonoids content as the dependent variable, the key factors that have a significant impact on the total flavonoids content of C. chine...

no. 3 example

[0041] The deep eutectic solution that 0.5g of turmeric powder is mixed with DES (choline chloride and ethylene glycol with a molar ratio of 1:2, ChCl-EG) that has the best extraction effect in the first embodiment of 10mL, Under optimal ultrasonic conditions (the water content in DES is 49.2%, the ultrasonic power is 300.6W, the ultrasonic time is 9.7 min, and the ultrasonic temperature is 40°C), adopt the method of step (2) of the first embodiment to obtain the extract of Caulis chinensis, record for DES-UAE.

[0042] The content of total flavonoids in the extract of P. chinensis was determined by the aluminum nitrate-sodium nitrite colorimetric method, and the phenolic substances were further identified and quantified by ultra-high performance liquid chromatography-Xevo triple quadrupole mass spectrometry. The conditions are as follows: Acquity UHPL CBEH-C18 chromatographic column (2.1 i.d.×100mm, 1.7 μm, Waters, USA), with 0.25% acetic acid aqueous solution (A) and 0.25% a...

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Abstract

The invention relates to the technical field of Chinese fevervine herb and root extraction, and particularly discloses an extraction method of phenolic substances of Chinese fevervine herb and root as well as an application of the extraction method. The method comprises the following steps that (1), Chinese fevervine herb and root powder is mixed with a deep eutectic solution, and ultrasonic treatment is performed to obtain a crude extracting solution; and a deep eutectic solvent is any one of a combination of choline chloride and ethylene glycol, a combination of choline chloride and triethylene glycol, a combination of choline chloride and glycerol, and a combination of L-proline and ethylene glycol. and (2), solid substances in the crude extracting solution are removed, and clear liquid is taken to obtain an extracting solution containing the phenolic substances. Compared with a conventional extraction method, the method for extracting the phenolic compounds in the Chinese fevervine herb and root by adopting a deep eutectic solvent coupling ultrasonic-assisted extraction process is higher in phenolic substance content and antioxidant activity and is more environment-friendly, and the obtained phenolic substances can be used for preparing antioxidant active drugs, for regulating the immune function and for preventing chronic diseases.

Description

technical field [0001] The present invention relates to the technical field of extracting vine dung, in particular to a method for extracting phenolic substances from chrysanthemum and its application. Background technique [0002] The information disclosed in the background of the present invention is only intended to increase the understanding of the general background of the present invention, and is not necessarily to be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Cockpit is a plant of the Rubiaceae genus Cauliflower, which mainly grows in southern China, Vietnam, Japan and India. It has been used as a traditional Chinese medicine for treating indigestion, jaundice, pain and dysentery, and has also been used in many local traditional foods for a long time production. Modern pharmacological experiments have identified phytochemical components such as iri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61K47/18A61K47/10A61P39/06A61P37/02A61P43/00A61K135/00A61K127/00
CPCA61K36/74A61K47/186A61K47/10A61P39/06A61P37/02A61P43/00A61K2236/333A61K2236/53
Inventor 肖娟刘雨欣郭志强
Owner HAINAN UNIVERSITY
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