Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

RAPD reaction system and kit for euonymus bungeanus

A reaction system, the technology of peach leaf eucalyptus, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problem that the repeatability and stability of the experimental results are difficult to guarantee, there is no unified reaction conditions and reaction system, and there is no universality. issues of sex

Active Publication Date: 2021-09-14
YANBIAN UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, RAPD technology has been widely used in plants such as wheat, star grass, fufangteng, pomegranate, peanut, etc., but it is rarely used in Euonymus peach leaf. At the same time, RAPD molecular markers also have some shortcomings, such as RAPD reaction does not The uniform reaction conditions and reaction system are not universal, and the RAPD reaction is easily affected by the experimental conditions, so it is difficult to guarantee the repeatability and stability of the experimental results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • RAPD reaction system and kit for euonymus bungeanus
  • RAPD reaction system and kit for euonymus bungeanus
  • RAPD reaction system and kit for euonymus bungeanus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Experimental method

[0031] (1) Extraction and detection of genomic DNA

[0032] Collect 10 fresh Euonymus euonymus leaves from Longjing Nursery of Jilin Province and Chaoyangchuan International Airport Nursery Base and adopt the modified urea method (Zheng D H, Van K, Lee S H. Molecular diversity and relationships among elite maize inbreds from US and CIMMYT populations and currenttheterotic groups in China[J].Hereditas,2010,145(4):182-193) extracted Euonymus persicae genomic DNA, and used agarose gel electrophoresis to detect the quality of Euonymus persicae genomic DNA.

[0033] (2) Screening of primers

[0034] OPA-16 was selected as the primer, and the nucleotide sequence of the OPA-16 was AGCCAGCGAA (SEQ ID NO.1) to amplify the genomic DNA of Euonymus persicae.

[0035] (3) Optimization of RAPD reaction system

[0036] The RAPD reaction system was determined to be 20 μL. In order to determine the optimal conditions for the RAPD reaction of Euonymus peach leaf...

Embodiment 2

[0060] Application example of RAPD reaction system of Euonymus peach leaf

[0061] Based on the optimal reaction system of Euonymus persicae RAPD described in Example 1, OPA-16 is used as a primer, and the nucleotide sequence of OPA-16 is AGCCAGCGAA (SEQ ID NO.1), according to Example 1 The specific steps of the PCR amplification and the detection of the amplified products are to amplify the genomic DNA of 10 copies of Euonymus peach leaf young leaves.

[0062] Depend on Figure 7 It can be seen that all 10 young leaves of Euonymus persicae were effectively amplified, and the amplified bands were clear and complete, that is, the established RAPD reaction system of Euonymus persicae was stable and suitable for the research of Euonymus persicae.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an RAPD reaction system for euonymus bungeanus, and belongs to the technical field of biological molecular markers. The total amount of the RAPD reaction system is 20 [mu] L, and the RAPD reaction system comprises the following components of 25-30 ng of template DNA, 0.15-0.25 [mu] mol / L of a primer, 2.50-2.70 mmol / L of MgCl2, 0.10-0.15 mmol / L of dNTP, 1.75-2.50 U of Taq DNA polymerase, and the balance of ddH2O. A strip amplified by the RAPD reaction system for the euonymus bungeanus is clear, complete and high in stability, and the blank of research on genetic diversity of the euonymus bungeanus in China is filled.

Description

technical field [0001] The invention relates to the technical field of biological molecular markers, in particular to a RAPD reaction system and a kit of Euonymus persicae. Background technique [0002] Euonymus bungeanus Maxim., also known as silk cotton wood, Mingkai Yehe, and Baidu, is a small deciduous tree of the genus Euonymus L. in the family Celastraceae. Euonymus bungeanus Maxim. It is an excellent landscaping tree species. In addition, the wood of Euonymus peach leaf is a high-quality material for making utensils and dense carvings. The bark contains hard rubber, and the oil content of the seeds is as high as 40%. The leaves can replace tea, and the flowers, fruits and roots can be used as medicine. Gan Ping can treat symptoms such as restlessness, boredom, insomnia, lung abscess, swelling, fracture, so Euonymus peach leaf can be used as a kind of high-quality economic tree species again. [0003] Randomly amplified polymorphic DNA (Random Amplified Polymorphic D...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2535/139C12Q2531/113
Inventor 赵成日李玉洁曹后男刘继生金美玉霍嘉鋆崔慕雪崔凯歌张雪
Owner YANBIAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com