Additive for improving in-vitro maturation quality of oocytes, culture method and application

Abstract

The invention discloses an additive for improving the in-vitro maturation quality of oocytes. The in-vitro culture quality of the oocytes is improved by adding a PF4 protein additive to an in-vitro oocyte culture solution. By improving the in-vitro culture quality of the oocytes, the additive can be applied to the fields of in-vitro maturation of the oocytes, somatic cell cloning technology and parthenogenetic embryos. On one hand, in-vitro maturation of the oocytes of various species can be promoted, in-vitro fertilized embryos and cloned embryos with better quality can be prepared, and in-vitro maturation culture of the oocytes of human assisted reproduction is facilitated. On the other hand, the prepared high-quality embryos and animals produced by the embryos are used for research in the fields of medicine, life science, animal husbandry and the like, and development of production practice is further promoted.

Description

technical field

[0001] The invention relates to an in vitro culture technology of oocytes, in particular to an additive for improving the quality of in vitro maturation of oocytes, a culture method and application. Background technique

[0002] Oocyte in vitro maturation (in vitro maturation, IVM) technology refers to taking immature oocytes from ovarian follicles, placing them in in vitro maturation medium for culture, and simulating the environment of follicles in vivo as much as possible to make them mature And gain the ability to fertilize. This technology has very important application value in the fields of life science, medicine, human assisted reproduction and animal husbandry, and is a basic technology for obtaining a large number of mature oocytes. In the actual production of animal husbandry, mature oocytes are used as recipient cells, somatic cells of animals with excellent traits are used as donor cells, and then a large number of excellent oocytes with the sam...

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