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Standardized production method for large-scale breeding of hevea brasiliensis somatic embryo seedlings

A production method and technology of rubber trees, applied in the field of plant tissue culture, can solve problems such as the inability to meet large-scale and high-efficiency factory production, lack of factory production mode, and aging of culture materials, so as to achieve adjustable and controllable production progress and facilitate production arrangements The effect of operation and convenient arrangement of production

Active Publication Date: 2021-10-01
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method only provides a technical route and lacks a factory production model
The production method of breeding rubber tree body embryo seedlings explored by this technical route has the problems of aging culture materials and waste of resources, and the production efficiency is low and the production cost is high, which cannot meet the needs of large-scale and high-efficiency production in factories.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Inoculated workers cycle production mode

[0052] Taking the weekly working time of the optimal cycle time according to the present invention as an example, there are 5 working days per week and 8 working hours per working day, so the cycle time β=5 days (40 hours). According to the above preconditions, from the cycle time calculation equation β=α+x*α+(z-1)*y*α, it can be calculated that α=1 day (8 hours). Arrange for 1 day and 3 days for seedling emergence. Therefore, the embryos are cut for 1 day and the seedlings emerge for 3 days each week, and each inoculation worker can cut 160 somatic embryos per week, and 3*160*3 = 1440 somatic embryos for emergence. A total of 50*1440=72,000 somatic embryos can emerge every week by 50 inoculation workers, and a total of 72,000*70%=50,400 test-tube seedlings can be grown. 50 inoculation workers produce 50 weeks a year, and can produce 50*50,400 = 2.52 million test-tube seedlings in total. Each inoculating worker can...

Embodiment 2

[0057] Example 2: Cooperative circulation production mode of inoculation team

[0058] Take the optimal number of labor for cutting embryos in the present invention as 1 labor as an example. According to the above preconditions, the total number of people in the group can be calculated by the equation γ=α+x*α+(z-1)*y*α, and the total number of people in the group γ=5 labors. The 50 inoculation workers can be divided into 10 groups in total, and each group has 5 inoculation workers. Each group needs to arrange 1 labor for endo-embryo cutting and embryo emergence, and 3 labor for seedling emergence. Therefore, 1 labor for cutting embryos and 10 labor for the whole year. Embryos are produced throughout the year, and 30 human workers are employed to produce seedlings throughout the year. 3*160 = 480 somatic embryos emerge every day for each seedling worker, 30*480 = 14400 somatic embryos for 30 seedling workers per day, 50 weeks a year, 5 days a week, a total of 50*5*14400 seedli...

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Abstract

The invention provides a standardized production method for large-scale breeding of hevea brasiliensis somatic embryo seedlings, and relates to the technical field of plant tissue culture. Based on a secondary somatic embryogenesis technology, the steps and sequence of a somatic embryo seedling breeding technology are analyzed; labor hours or the number of workers required by each breeding step and a step interval period are identified; a secondary embryo multiplication coefficient of a certain variety of hevea brasiliensis is determined; and finally, according to a target demand, an optimal strategy under a production mode and standardized production is determined. The method starts from the direction of a large-scale production mode, solves the problems of lack of a hevea brasiliensis somatic embryo seedling factory production mode, culture material aging and resource waste, realizes controllable production progress, improves production efficiency, reduces resource waste, reduces production cost, and optimizes human resource allocation.

Description

technical field [0001] The present application provides a standardized production method for large-scale breeding of rubber tree embryos, and relates to the technical field of plant tissue culture. Background technique [0002] Rubber tree [Hevea brasiliensis (Willd.ex A.Juss.)Muell.Arg.] is a plant of Euphorbiaceae, a perennial tropical tall tree in the upper layer of tropical rainforest, with an economic lifespan of up to 30-40 years, and the latex secreted is important. Moreover, the wood material of rubber tree is light, the pattern is beautiful, and the processing performance is good. After chemical treatment, it can be used to make high-grade furniture, fiberboard, plywood, pulp, etc., which has high economic value. [0003] At present, the mainstream seedling cultivation method is self-rooted juvenile clonal propagation technology, which has the excellent effects of fast growth, high yield and high resistance. Specifically, this technology mainly obtains regenerated ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 顾晓川吴日智华玉伟黄华孙黄天带成镜彭素娜徐正伟戴雪梅周权男李季
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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