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Kit and detection method for detecting mycoplasma pneumoniae nucleic acid and drug resistance gene variation of mycoplasma pneumoniae nucleic acid

A technology of Mycoplasma pneumoniae and drug-resistant genes, applied in the field of molecular biology, can solve problems such as limited sensitivity, achieve the effect of shortening the reaction time and preventing residual pollution

Pending Publication Date: 2021-10-01
TOYOBO CO LTD
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Problems solved by technology

Considering that the concentration of Mp in the patient's sputum sample is about 10 2 ~10 6 cfu / ml, while the detectable concentration range of antigen detection technology is 10-100cfu / ml, the sensitivity of antigen detection without Mp amplification and culture is very limited, so when nucleic acid amplification technology can be used for diagnosis, antigen detection is not recommended to diagnose

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  • Kit and detection method for detecting mycoplasma pneumoniae nucleic acid and drug resistance gene variation of mycoplasma pneumoniae nucleic acid
  • Kit and detection method for detecting mycoplasma pneumoniae nucleic acid and drug resistance gene variation of mycoplasma pneumoniae nucleic acid
  • Kit and detection method for detecting mycoplasma pneumoniae nucleic acid and drug resistance gene variation of mycoplasma pneumoniae nucleic acid

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Embodiment

[0064] Embodiments, detection kits and detection methods for Mycoplasma pneumoniae nucleic acid and the presence or absence of drug-resistant gene mutations

[0065] The wild-type base sequence of the Mycoplasma pneumoniae nucleic acid 23S rRNA gene that can be a detection object of the present invention is the base sequence shown in the sequence number 33 in the sequence listing.

[0066] The detection kit for Mycoplasma pneumoniae nucleic acid and its drug-resistant gene variation includes KOD DNA polymerase, primer MPN-F, primer MPN-R, and specific targeting fluorescent probes,

[0067] The aforementioned primer MPN-F is a continuous sequence in the base sequence shown in No. 1910 to No. 2039 of the Mycoplasma pneumoniae nucleic acid 23S rRNA gene (SEQ ID NO: 33) or in a base sequence complementary thereto and has a length of 25 to 36 A forward primer consisting of a nucleotide sequence of 1 base,

[0068] The aforementioned primer MPN-R is a continuous sequence of 25 to 3...

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Abstract

The invention provides a kit and a detection method for detecting mycoplasma pneumoniae nucleic acid and drug-resistant gene variation, which are beneficial for detecting the mycoplasma pneumoniae nucleic acid and drug-resistant gene variation of the mycoplasma pneumoniae nucleic acid and the drug-resistant gene variation of the mycoplasma pneumoniae nucleic acid. The kit and the detection method for detecting mycoplasma pneumoniae nucleic acid and drug-resistant gene variation of the mycoplasma pneumoniae nucleic acid are characterized in that the kit comprises KOD DNA polymerase, a primer MPN-F, a primer MPN-R and a specific targeting fluorescent probe. According to the kit and the detection method disclosed by the invention, the aim of rapidly, simply, conveniently and accurately detecting the mutations of the mycoplasma pneumoniae nucleic acid, the 23S rRNA gene of the mycoplasma pneumoniae nucleic acid and the 2063th or 2064th gene locus of the 23SrRNA is fulfilled by designing the primers and the probes with specific sequences.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular, to a detection kit and a detection method for Mycoplasma pneumoniae nucleic acid and its drug-resistant gene variation. The kit is used to qualitatively detect sputum samples, throat swab samples, or The Mycoplasma pneumoniae 23S rRNA gene and its variation in nasal swab samples are suitable for auxiliary diagnosis of Mycoplasma pneumoniae infection. Background technique [0002] Mycoplasma pneumoniae (M Pneumonia, also referred to as MP or Mp) is the pathogen of human mycoplasma pneumonia, which is mainly transmitted by droplets, with an incubation period of 2 to 3 weeks. At present, Mycoplasma pneumoniae has become one of the main pathogens of community-acquired pneumonia in children, and it is also a common pathogen of respiratory tract infections in adolescents and elderly people. MP is sensitive to macrolide antibiotics that inhibit microbial protein synthesis, quin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6858C12Q1/04C12R1/35
CPCC12Q1/689C12Q1/6858C12Q2600/156C12Q2531/113C12Q2563/107C12Q2527/107
Inventor 川岛洋介
Owner TOYOBO CO LTD
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