CYP3C19*3 genotype detection kit and CYP3C19*3 genotype detection method

Abstract

The invention discloses a CYP3C19*3 genotype detection kit and a CYP3C19*3 genotype detection method. The kit is characterized in that the kit comprises KOD DNA polymerase, an upstream primer, a downstream primer, a specific targeting fluorescent probe, a GG type positive reference product, an AA type positive reference product and a GA type positive reference product; a GG homozygous DNA sequence is a linear recombinant DNA sequence designed by using the GG type in the 636 locus on a human CYP3C19*3 gene as a template; an AA homozygous DNA sequence is a linear recombinant DNA sequence designed by using the AA type in the 636 locus on the human CYP3C19*3 gene as a template; and the GA type positive reference product is prepared through mixing the GG type positive reference product with the AA type positive reference product according to a volume ratio of 1:1. The detection method allows the variation of the CYP2C19*3 locus on the CYP2C19 gene to be quickly and easily detected.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a CYP2C19*3 genotype detection kit and a detection method thereof. The kit adopts the PCR in vitro amplification method and is used for the qualitative detection of drug-metabolizing enzyme CYP2C19* in human whole blood samples The variation of the 636th position of the 3 positions. Background technique [0002] Cytochrome P450 (Cytochrome P45, referred to as CYP) isoenzymes, also known as drug enzymes, is a superfamily composed of a series of structurally and functionally related enzymes, and is the main enzyme system for drug metabolism in vivo. Among them, the CYP2C19 enzyme has genetic polymorphism, and the enzyme activity has significant differences among different individuals. The efficacy and side effects of drugs metabolized by CYP2C19 enzymes also vary significantly depending on the patient's genotype. CYP2C19 is involved in the metabolism of drugs such as cl...

AI Technical Summary

Problems solved by technology

Main disadvantages: low sensitivity, especially in the detection of somatic mutations in tumor tissue, when the target gene mutation ratio in the tissue is less than 20%, false negative results may occur; there are special requirements for reagents and instruments, and it is not easy to popularize; operation Complex, relatively high cost, slow speed, low throughput

Main disadvantages: There are special requirements for reagents and instruments, and it is not easy to popularize; the detection sensitivity is limited, and it is prone to false negatives for low-abundance somatic mutations (<3%) in tumor tissues; the sequencing length is only more than 10 bases, and cannot be detected Analysis of long fragments

This method belongs to qualitative detection, and can only be used to detect known polymorphism sites, and cannot identify unknown polymorphisms

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