ADRB1 G1165C genotype detection kit and detection method thereof

Abstract

The invention relates to an ADRB1 G1165C genotype detection kit and a detection method thereof. the ADRB1 G1165C genotype detection kit is characterized by comprising KOD DNA polymerase, an upstream primer, a downstream primer, a specific targeting fluorescent probe, a GG type positive reference substance, a CC type positive reference substance and a GC type positive reference substance; the GG homozygous DNA sequence is a linear recombinant DNA sequence designed by taking the GG type at the 1165 site of the human ADRB1 gene as a template; the CC homozygous DNA sequence is a linear recombinantDNA sequence designed by taking the CC type at the 1165 site of the human ADRB1 gene as a template; the GC type positive reference substance is prepared by mixing the GG type positive reference substance and the CC type positive reference substance according to equal volume. The detection method of the ADRB1 G1165C genotype detection kit can quickly and conveniently detect the mutation of the 1165 site of the ADRB1 gene.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to an ADRB1 G1165C genotype detection kit and a detection method thereof. The kit adopts the PCR in vitro amplification method and is used for qualitatively detecting the 1165th site of ADRB1 in human whole blood samples. genetic polymorphism. Background technique [0002] Genetic factors are one of the important reasons for the occurrence and development of diseases and racial and individual differences in drug response. Human genes encoding drug response-related proteins (including transporters, drug metabolizing enzymes, and drug targets) have genetic polymorphisms. This polymorphism can lead to a change in the function of the corresponding protein it encodes. In recent years, it has been found that some drug-related proteins in intracellular signal transduction pathways have polymorphisms, which lead to functional variation of proteins, and then change the reactivity ...

AI Technical Summary

Problems solved by technology

Main disadvantages: low sensitivity, especially in the detection of somatic mutations in tumor tissue, when the target gene mutation ratio in the tissue is less than 20%, false negative results may occur; there are special requirements for reagents and instruments, and it is not easy to popularize; operation Complex, relatively high cost, slow speed, low throughput

Main disadvantages: There are special requirements for reagents and instruments, and it is not easy to popularize; the detection sensitivity is limited, and it is prone to false negatives for low-abundance somatic mutations (<3%) in tumor tissues; the sequencing length is only more than 10 bases, and cannot be detected Analysis of long fragments

When the molecular weight marker reaction tube has no bands or weak bands, the possible reasons include leaking wells, insufficient or invalid fluorescent dyes, too long electrophoresis time or too high voltage

The advantage of this method is that it is low in cost and can be carried out in ordinary laboratories; the disadvantage is that it is only suitable for qualitative determination of DNA insertion / deletion polymorphisms or fusion genes, and cannot be used for SNP detection

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