Characteristic polypeptide for identifying deer-horn glue of Cervus nippon or Cervus elaphus, and application of characteristic polypeptide

A technology of sika deer and antler glue, applied in the fields of peptides, instruments, analytical materials, etc., can solve the problems of less research data, less research materials, and lack of protein databases on collagen and keratin, and achieve excellent specificity and stability. The effect of strong specificity and good application prospect

Inactive Publication Date: 2021-10-08
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology provides two ways to identify animals that have been infected with diseases such as Lyme disease (Lyme borreliosis). One way it uses specialized proteins called unique sequences from certain bacteria found on their surfaces. Another approach involves comparing these patterns between different individuals' skirts made up of similar structures like those seen during previous experiments. These techniques are useful because they allow researchers to easily determine which animal species may transmit this illnesses through blood transfusion medicine.

Problems solved by technology

This patented describes an issue where identifying specific types of antills from Chinese pharmaceuticals such as pearlled skunks (PZ) remains challenges because they lack unique characteristics that make them hard to detect even if their quality suffices. Current methods involve searching databases containing many samples and comparing sequences based upon these properties alone, but this approach requires significant effort and time compared to studying biological systems like birds without horses.

Method used

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  • Characteristic polypeptide for identifying deer-horn glue of Cervus nippon or Cervus elaphus, and application of characteristic polypeptide
  • Characteristic polypeptide for identifying deer-horn glue of Cervus nippon or Cervus elaphus, and application of characteristic polypeptide
  • Characteristic polypeptide for identifying deer-horn glue of Cervus nippon or Cervus elaphus, and application of characteristic polypeptide

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Experimental program
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Embodiment 1

[0030] 1, measure conditions

[0031] 1.1 Nanasheng liquid phase - high resolution mass spectrometry conditions

[0032] Na Raise Conditions: Separation of thermo Acclaim Pepmap C18 (100 μm × 3.5 cm, 5 μm), Isolation of thermo AccLaim Pepmap C18 (75μm × 15cm, 3 μm), flow rate 300nl / min, mobile phase A is 2 The 0.1% aqueous formation of% acetonitrile, the mobile phase B is a 5.1% aqueous formic acid containing 98% acetonitrile, and gradient elution (0 ~ 1 min, 1% B → 6% B; 1 to 96 min, 6% B → 22% B) 96 ~ 113min, 22% B → 30% B; 113 to 117 min, 30% B → 95% B; 117 to 120 min, 95% B) were 1 μm. High scoretric spectrometry: Ion source is NanoSpray Flex njun. Analysis in positive ion mode, the spray voltage is 1800 V, the ion-transport capillary temperature is 275 ° C, and the S-Lens transport efficiency is set to 60%. The primary mass spectrum is used as a mass analyzer with a resolution of 60000. The acquisition range is from 350 to 1550 (m / z); the secondary mass spectrum is used a...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a characteristic polypeptide for identifying the deer-horn glue of Cervus nippon or Cervus elaphus, and application of the characteristic polypeptide. The characteristic polypeptide provided by the invention is as follows: a peptide fragment 1 is as shown in SEQ ID No. 1: ASTGAALVVR; and a peptide fragment 2 is as shown in SEQ ID No. 2: TPVGQGPSVPVPNR. The characteristic polypeptide and the detection method provided by the invention provide reference for searching a characteristic peptide fragment in related species lacking in a database. The characteristic polypeptide provided by the invention has excellent specificity and stability for the deer-horn glue of the Cervus nippon or the Cervus elaphus, is high in specificity, can be used for identifying deer-horn glue medicinal materials and Chinese patent medicines added with the the deer-horn glue medicinal materials, and has a good application prospect.

Description

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Claims

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Application Information

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Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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