Phascolarctobacterium faecium for use in the prevention and treatment of obesity and its comorbidities
A technology of strains and uses, applied in the pharmaceutical and food fields, can solve the problems of no provision, reduction of tubular bacteria, no provision, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0085] Example 1. Separation and identification of bacterial strain P.faecium G104 (P.Faecium DSM 32890)
[0086] Different intestinal bacteria are separated from the feces of healthy volunteers. 1.25 g was diluted with 10 mM phosphate buffer containing 0.05% cysteine (1: 10 dilution), wherein the NaCl concentration was 130 mm (PBS) and in Lab-Blender Stomacher 400 (Seward Medical, London, Homogenization in 35uk. The dilute solution was inoculated in 37.5 ml of small intestinal bacteria medium (IBM), which was based on the medium recommended by previous publications (Gibson, GR, etal., Appl.environ MiCrobiol., 54 (1 1): 2750-5, 1988; Lesmes, u et al., J.agric.foodchem., 56: 5415-5421, 2008), and inventors have designed some modifications:
[0087] ■ Main ingredients: Distilled water (1600 mL), protein water (4 g), sodium bicarbonate (4 g), calcium chloride (0.02 g), pectin (4 g), xylosan (4 grams), wheat bran Extract (4 g), Arabic hemarylose (2g), Arabic (2 g), starch (10 g), ca...
Embodiment 2
[0102] Example 2. Select P. Faecium based on its ability to regulate inflammation in vitro.
[0103] In vitro assays to compare the immunoassay characteristics of human intestinal sources bacteria, and select a strain capable of inducing maximum anti-inflammatory responses in classic monocytes, therefore has potential treatment value in the treatment of obesity-related inflammation. For this reason, the cell suspension of different bacterial strains is used as irritation in peripheral blood mononuclear cells (PBMC) culture, and the number of classic mononuclear cells is measured by flow cytometry and anti-inflammatory cytokines IL-4 relative to promotion. Level of inflammatory cytokine IFNγ.
[0104] Culture and stimulation of PBMC.
[0105] From the full blood of healthy volunteers, peripheral blood mononuclear cells (PBMC) were separated using FICOLL PAQUE-PLUS 17-1440-02, Bioscience. After dissolving red blood cells (red blood cell lysis buffer, RBC, Miltenyi Biotec. Gibco, Sp...
Embodiment 3
[0112] Example 3. Role of P.Faecium in the obese animal model
[0113] Development of obesity and sampling animal model.
[0114] Adult male C57BL / 6 mice (6-8 weeks, Charles River, Les Oncins, FRANCE) were held in controlled temperature (23 ° C), relative humidity (40-50%) and 12 hours of light / dark cycle conditions High fat (45% kcal) High sugar (sucrose) (17% kcal) High-calorie diet (HFHSD; D12451, study diet, BROGAARDEN, Denmark) or control diet (CD, 10% Kcal from fat, no sucrose; D12450K To study diet, BROGAARDEN, Denmark 14 weeks. Each day, mice feeding HFHSD diet are subject to the bacterial strain of the invention in 10% degreased milk [(1 × 10) 7 -1 × 10 -8 ) Colony forming unit (CFU)]. In the same manner, the carrier (10% degreasing milk) was administered to the control group and the slope (CD) control group (N = 10 mice per group). After 14 weeks, mice were killed by cervical spine, taking blood, intestines, liver, brain, groin and epididymis, white adipose tissue, ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More