Efficient watermelon genetic transformation system and application

A technology of genetic transformation, watermelon, applied in the field of plant genetic engineering

Active Publication Date: 2021-10-26
PEKING UNIV INST OF ADVANCED AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is still unknown whether these genes can improve the transformation efficiency in watermelon. Therefore, it is an urgent problem for those skilled in the art to provide a method for utilizing transgenic technology to improve the transformation efficiency of watermelon materials.

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  • Efficient watermelon genetic transformation system and application
  • Efficient watermelon genetic transformation system and application
  • Efficient watermelon genetic transformation system and application

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Embodiment Construction

[0022] In the present invention, unless otherwise specified, the scientific and technical terms used herein have the meanings commonly understood by those skilled in the art. Moreover, the terms related to protein and nucleic acid chemistry, molecular biology, cell and tissue culture, microbiology, immunology and laboratory operation steps used herein are all terms and routine procedures widely used in the corresponding fields. For example, standard recombinant DNA and molecular cloning techniques used in the present invention are well known to those skilled in the art and are described more fully in: Sambrook, J., Fritsch, E.F. and Maniatis, T., Molecular Cloning: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, 1989 (hereinafter "Sambrook"). Meanwhile, in order to better understand the present invention, definitions and explanations of related terms are provided below.

[0023] As used herein, the term "plant" includes the whole plant and any pr...

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Abstract

The invention relates to the field of plant genetic engineering. Specifically, the invention relates to an efficient watermelon genetic transformation system and application. Growth regulation genes such as AtGRF5, TaGRF4, OsGIF1, ZmWUS and ZmBBM are included in watermelon genetic transformation. It is found for the first time that expression of AtGRF5, TaGRF4, OsGIF1, ZmWUS or ZmBBM genes in the watermelon transformation and/or regeneration process can promote the genetic transformation efficiency of watermelon crops, AtGRF5 can significantly improve the genetic transformation efficiency of watermelons by about 80 times, and precious gene resources are provided for genetic breeding. The invention further provides a method for using a fluorescent protein DsRed2 as a genetic transformation screening marker, and genetic transformation screening operation is simplified. When the system is expressed and used in watermelons, the genetic transformation breeding efficiency of the watermelons can be effectively improved, and the breeding cost is reduced.

Description

technical field [0001] The invention provides a system and application for improving the genetic transformation efficiency of watermelons, specifically including genes, recombinant vectors, transformation methods, applications and identification methods of transgenic plants, belonging to the field of plant genetic engineering. Background technique [0002] Watermelon is an important economic crop all over the world, with a wide cultivation area and a large cultivation area. At present, the whole genome and pan-genome sequencing of watermelon have been completed, and many potential regulatory genes that may regulate watermelon yield, nutritional quality, fruit shape, disease resistance and stress tolerance have been discovered. However, many traits cannot be improved through conventional breeding methods, and gene function verification and variety improvement need to be completed through genetic engineering technology. With the continuous development of biotechnology, the ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84C12N15/65A01H5/00A01H6/34
CPCC12N15/8205C12N15/8212C07K14/415
Inventor 张华伟章旺根潘文波程振涛
Owner PEKING UNIV INST OF ADVANCED AGRI SCI
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