Thionine labeled antibody immunochromatography testing strip for visually and rapidly detecting transgenic protein

Abstract

The invention relates to a thionine labeled antibody immunochromatography testing strip for visually and rapidly detecting transgenic protein, the thionine labeled antibody immunochromatography testing strip comprises a bottom plate, and a sample pad, a combination pad, a nitrocellulose membrane and absorbent paper which are fixed on the bottom plate and are connected in sequence, and a labeled antibody labeled by thionine is fixed in the combination pad; a Thi-mAb1 compound which is formed by coupling the thionine with a monoclonal antibody mAb1 through glutaraldehyde. The immunochromatography testing strip provided by the invention takes Thi as a signal marker, does not need a nano material, does not need a complex synthesis process, utilizes a conventional cross-linking agent to be rapidly coupled with an antibody through a covalent bond, breaks through the tedious preparation process of colloidal gold, greatly shortens the time for marking the antibody, simplifies the preparation process, and can rapidly detect transgenic protein; the sensitivity is high; and the specificity, the stability and the reproducibility are good.

Description

technical field [0001] The invention belongs to the technical field of rapid immunodetection, in particular to a thionine-labeled antibody immunochromatographic test strip for visual and rapid detection of transgenic proteins. Background technique [0002] At present, more and more detection techniques are applied to the detection of genetically modified crops. PCR is the main method to detect the specific DNA of transgenic crops, but its operation is cumbersome, time-consuming and requires expensive instruments, which is not suitable for on-site detection. LAMP and RPA overcome the drawbacks of PCR and can be detected quickly, sensitively, and with high specificity. However, they use agar gel electrophoresis, which is time-consuming and easy to contaminate, so there are still certain limitations. [0003] Although the above detection based on nucleic acid level has ultra-high sensitivity, there are still certain disadvantages in the actual sample on-site detection. The de...

AI Technical Summary

Problems solved by technology

The continuous update of markers improves the detection sensitivity of ICS, but most of these materials require complex synthesis and modification processes

[0005] Nanomaterials such as colloidal gold are widely used in the construction of test strips as color signals, but complex antibody labeling and material modification processes are required, and the sensitivity is generally low. At the same time, in the actual detection of traditional colloidal gold test strips, It is easily affected by the sample matrix, such as blood and blood-stained samples, and has certain limitations; there are also many fluorescent materials, such as cadmium telluride quantum dots and up-conversion nanomaterials, which are expensive and need to be visualized with a small light source

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