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CircGLS2 and application thereof in diagnosis, treatment and prognosis of liver cancer

A liver cancer, molecular technology, applied in the field of biomedicine, to achieve the effect of weight and volume increase, weight and volume reduction

Active Publication Date: 2021-11-26
THE NAVAL MEDICAL UNIV OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004]However, whether circRNA may be a potential biomarker or therapeutic target, and its role in the occurrence and recurrence of HCC has not been reported yet

Method used

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  • CircGLS2 and application thereof in diagnosis, treatment and prognosis of liver cancer
  • CircGLS2 and application thereof in diagnosis, treatment and prognosis of liver cancer
  • CircGLS2 and application thereof in diagnosis, treatment and prognosis of liver cancer

Examples

Experimental program
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Effect test

Embodiment 1

[0071] A total of 13 patients who were pathologically diagnosed as HCC and underwent radical resection in Shanghai Oriental Hepatobiliary Surgery Hospital from February 2011 to September 2012 were selected as the research objects. All the enrolled patients did not receive any drug treatment, the surgical margin was 2 cm, and there was no intrahepatic or distant metastasis. Tumor tissues, paired recurrent tumor tissues and paired adjacent tissues were removed immediately after operation and stored at -80°C. Of the 13 patients, 4 relapsed within 2 years after the first curative resection, and 9 did not relapse within 5 years after the first radical resection. The mRNA, circRNA and microRNA (miRNA) expression profiles of the above 13 patients were sequenced and analyzed by multiple groups. The study protocol complied with the 1975 Declaration of Helsinki and was approved by the Ethics Committee of the Naval Medical University. Each participant obtained a signed informed consent...

Embodiment 2

[0074]A total of 110 patients who were pathologically diagnosed as HCC and underwent radical resection at Shanghai Oriental Hepatobiliary Surgery Hospital from February 2011 to September 2012 were selected as the validation cohort. All the enrolled patients did not receive any drug treatment, the surgical margin was 2 cm, and there was no intrahepatic or distant metastasis. Tumor tissues and adjacent tissues were removed immediately after operation and stored at -80°C. The study protocol complied with the 1975 Declaration of Helsinki and was approved by the Ethics Committee of the Naval Medical University. Each participant obtained a signed informed consent form.

[0075] During postoperative follow-up, we found that 52 of 110 patients relapsed. The expression levels of circGLS2 mRNA in different tissues of 110 patients were detected by real-time fluorescent quantitative PCR. The study found that the expression of circGLS2 in liver cancer tumor tissues was significantly low...

Embodiment 3

[0077] Combined with the prognosis information, the 110 patients in Example 2 were divided into a circGLS2 high expression group and a low expression group. R software (version 3.5.3) was used for survival analysis of patients. Kaplan-Meier method was used for survival curve analysis, and log-rank test was used for comparison between two groups. The results showed that the low expression of circGLS2 in the tumor tissue of liver cancer patients was closely related to the poor prognosis of the patients, and the disease-free survival time and overall survival time of patients with low expression were shorter than those with high expression (p=9.46e-3; p=2.64 e-5) (see Figure 4 ).

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Abstract

The invention relates to the technical field of biological medicine, and particularly provides a brand-new circular RNA molecule circGLS2, a recombinant vector containing the circGLS2, and application of the circGLS2 in diagnosis, treatment and prognosis evaluation of the liver cancer. The circGLS2 has the beneficial effects that the circGLS2 provided by the invention plays an important role in the aspect of inhibiting tumor generation and liver cancer recurrence, can be used as a novel biomarker for diagnosis, treatment and analysis prognosis of the liver cancer, meanwhile, can also be used as a novel potential treatment target spot, and can provide scientific, precise and personalized diagnosis and treatment schemes for liver cancer patients through the circGLS2 expression level in the body of the patients.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, specifically, circGLS2 and its application in the diagnosis, treatment and prognosis of liver cancer. Background technique [0002] In 2020, primary liver cancer was the third leading cause of cancer death worldwide. Hepatocellular carcinoma (HCC) accounts for 75% to 85% of primary liver cancers. Chronic inflammation caused by chronic hepatitis B and C virus infection and lifestyle-related insulin resistance / hyperinsulinemia are important causes of HCC. The onset of liver cancer is occult, and the vast majority of patients are already in the middle and late stage when they go to the doctor, and there is no effective radical treatment, and the prognosis is poor. Surgical resection, liver transplantation, and radiofrequency ablation of the liver are currently considered to be more effective treatments. However, due to the characteristics of easy metastasis and postoperative recurrence...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/85C12Q1/6886A61K31/7105A61P35/00
CPCC12N15/113C12N15/85C12Q1/6886A61P35/00C12N2310/532C12N2320/32C12N2800/107C12Q2600/158C12Q2600/178C12Q2600/118C12Q2600/136
Inventor 殷建华陈曦曹广文蒲蕊吴婷
Owner THE NAVAL MEDICAL UNIV OF PLA
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