Biological introduction aid and method of use thereof
A technology of organisms and adjuvants, applied in the field of compositions containing active substances, preventing and/or treating diseases, or compositions for improving physical fitness and/or physical conditions, can solve the problem of improving the efficiency of introduction, without evidence and other issues, to achieve the effect of improving physical fitness and/or physical condition
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[0329] Hereinafter, the present invention will be described in more detail using examples, comparative examples, etc., but the present invention is not limited by these examples.
[0330] In addition, before describing Examples etc., the "microorganism detection method" used in Examples etc. is demonstrated.
[0331] (Detection method of microorganisms)
[0332] The determination of the number and type of microorganisms was carried out by combining known "ribosome profiling (16S ribosomal RNA (rRNA) sequencing)" and known "next generation sequencer (Next Generation Sequencer: NGS)".
[0333] It should be noted that, as a profiling method, in addition to "ribosome profiling", "shotgun metagenomics sequence" that comprehensively analyzes the entire genome of all microorganisms present in the subject can also be used et al. have used "ribosome profiling" which can be performed at low cost with a small number of sequences.
[0334] (Ribosome Profiling)
[0335] The so-called "r...
Embodiment A
[0341] [Example A: Biointroduction Auxiliary A (Containing Air Bubbles Below Nano Size)]
[0342] "Materials and methods: Material and method"
[0343] (Manufacture of "Nano Bubble Water A")
[0344] Using the above-mentioned apparatus and the like, "biological body introduction auxiliary agent A (nanobubble water A)" of the present invention (Example A) was produced using mineral water and air as materials.
[0345] Using "Multisizer 3" manufactured by Beckman Coulter Co., Ltd., the "nanobubble water A" prepared above was diluted 250 times for the convenience of measuring the diameter of the bubbles, and the size and number of bubbles in the diluted solution were measured. show the result in figure 1 middle.
[0346] The analysis result of "nanobubble water A" is the uppermost line graph in the figure indicated by "*".
[0347] figure 1 Analysis results: average bubble diameter: 830nm, number of bubbles: about 435,000 / ml
[0348] Therefore, the number of bubbles of "nan...
Embodiment B
[0349] [Example B: Biointroduction Auxiliary B (Containing Air Bubbles Below Nano Size)]
[0350] "Materials and methods: Material and method"
[0351] As the gas to be sealed when the bubbles are generated, air and hydrogen are used together, and the timing of filling the hydrogen is in the second half of the filling, and the hydrogen concentration in the bubbles is increased. In the same manner as in Example A, a "Biointroduction auxiliary agent B (nanobubble water B)" of the present invention (Example B).
[0352] It should be noted that the amount of hydrogen gas used in the enclosing operation was about 250 times that of the atmosphere.
[0353] The number of bubbles of "nanobubble water B" (undiluted 250 times) used in the present invention is also considered to be about 100 million / ml.
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