A chondroitin sulfate polysaccharide probe based on upconversion nanomaterials and its preparation method and application

A technology of chondroitin sulfate and nanomaterials, which is applied in the field of probe synthesis, can solve the problems of weak up-conversion luminescence and long reaction time, and achieve the effect of enhanced luminous intensity and short reaction time

Active Publication Date: 2022-06-07
SHANDONG UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the preparation of this invention requires high pressure and high temperature in a reactor, and the reaction time is long, and the up-conversion luminescence is weak.

Method used

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  • A chondroitin sulfate polysaccharide probe based on upconversion nanomaterials and its preparation method and application
  • A chondroitin sulfate polysaccharide probe based on upconversion nanomaterials and its preparation method and application
  • A chondroitin sulfate polysaccharide probe based on upconversion nanomaterials and its preparation method and application

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Embodiment 1

[0060] A method for preparing a chondroitin sulfate polysaccharide probe based on up-conversion nanomaterials, comprising the steps of:

[0061] (1) Synthesis of CS-ADH

[0062] Accurately weigh 1 g of CS, dissolve it in 50 mL of water, add 4 g of EDC and 0.585 g of NHS, adjust the pH to about 6-7 with 0.1 mol / L HCl, and activate the carboxyl group in a water bath at 37 °C for 15 min; add 8.8 g of ADH, and use 0.1 mol of / L NaOH aqueous solution to adjust the pH to 6-7, after 24 hours of reaction in a water bath at 37 °C, dialyzed against water for three days (molecular weight cut-off = 4000 Da), freeze-dried to obtain CS-ADH solid, which was obtained by FTIR and 1 H NMR confirmed the coupling of CS to ADH.

[0063] figure 1 a and b are the infrared spectra of CS-ADH and CS, respectively. In the infrared spectrum of CS, the main observation is 1603.19cm -1 C=O characteristic stretching vibration peak of C=O; the C=O stretching vibration peak in CS-ADH appeared at 1649.71cm...

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Abstract

The invention provides a chondroitin sulfate polysaccharide probe based on an upconversion nanomaterial, a preparation method and application thereof. The present invention uses adipate dihydrazide (ADH) as a bridge to graft up-conversion nanomaterials on the molecular chain of chondroitin sulfate through the coupling of carboxyl amino groups, and through the tumor targeting effect of chondroitin sulfate And based on the luminescent properties of rare earth up-conversion materials, a chondroitin sulfate probe was constructed. Compared with the existing tumor imaging probes, due to the anti-Stokes luminescence characteristics of upconversion nanomaterials, tumor imaging in vivo in normal immune mice can be realized under 980nm excitation, with better biocompatibility and less background fluorescence interference Small and can be used for in vivo and in vitro imaging of tumors.

Description

technical field [0001] The invention relates to a chondroitin sulfate polysaccharide probe based on up-conversion nanomaterials, a preparation method and application thereof, and belongs to the technical field of probe synthesis. Background technique [0002] Liver cancer is the most common cause of cancer death in the world, and hepatocellular carcinoma is the most common type of liver cancer with high mortality and high incidence of distant metastasis. The occurrence and development of hepatocellular carcinoma (HCC) is a multi-step and multi-stage pathological process. The molecular mechanism of its pathogenesis is very complex, and the early symptoms are not obvious. The early diagnosis rate of hepatocellular carcinoma, the improvement of clinical efficacy and the prevention and treatment of hepatocellular carcinoma are of great significance. At present, the commonly used clinical methods for cancer diagnosis include X-ray diagnosis, positron emission tomography-computed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G83/00C08B37/08C09K11/02C09K11/85G01N21/01B82Y20/00B82Y30/00B82Y40/00
CPCC08G83/001C08B37/0069C09K11/02C09K11/7773G01N21/01B82Y20/00B82Y30/00B82Y40/00Y02P20/55
Inventor 刘秀美巩洁
Owner SHANDONG UNIV
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