Regeneration medium for ramie tissue culture and culture method and application thereof

Abstract

The invention discloses a regeneration medium for ramie tissue culture and a culture method and application thereof. The regeneration medium comprises a bud induction culture medium for inducing ramie explants to form adventitious buds, the bud induction culture medium is prepared from MS, 0.32-0.45 mg / L of TDZ, 0.025-0.036 mg / L of LIAA, 135-220 mg / L of casein hydrolysate, 25-36 g / L of sucrose and 6-8.5 g / L of agar, and the pH value of the bud induction culture medium is 5.5-6.0. By optimizing the components of the regeneration medium, the regeneration efficiency can be improved, and the bud number and the seedling emergence number of the explant are increased; and by adjusting the tissue culture method, callus induction and bud differentiation are completed at a time, transfer and culture medium replacement are not needed, the production cost is reduced, the tissue culture period is shortened, the tissue culture procedure is simplified, and the regeneration medium has good application prospects in ramie molecular breeding and germplasm resource preservation.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and more particularly relates to a regeneration medium for ramie tissue culture, a culture method and application thereof. Background technique [0002] Ramie is a perennial herb of the Urticaceae (Urticaceae) genus Boehmeria, and is a traditional textile fiber crop in my country; the ramie fiber has high strength, long fiber, good hygroscopicity and hygroscopicity, and has antiseptic and antibacterial functions at the same time. It is an extremely important raw material in the textile industry. [0003] Ramie is monoecious and has different flowers, the seeds are extremely small, and the plants are all heterozygous. There are still problems such as character segregation and genetic variation when seedlings are raised, and it is difficult to maintain the excellent traits of the female parent line. [0004] In the improvement of ramie varieties, conventional breeding methods such as s...

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Problems solved by technology

[0005] In the prior art, the tissue culture of ramie has been reported, and the regenerated shoots can be induced successfully, but the differentiation rate and the shoots differentiated by each piece of callus are very few, and the process is cumbersome, requiring callus induction and sprout differentiation In the two steps, two different media are used respectively, and the callus induction link needs to be subcultured every few days, which not only consumes labor, increases the cost of medicines, and is also likely to cause pollution during the transfer process of different media; and , It takes a long time to obtain differentiated seedlings that can be used for rooting, and it takes more than 45 days (20-30 days for callus induction + 25-30 days for seedling differentiation)

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